Phosphate Buffered Saline: 1 L of 20X is a concentrated buffer solution ready for dilution to a 1X Phosphate-buffered saline (PBS) working solution commonly used in biological research. Phosphate Buffered Saline is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption. Additional sizes, formats, and concentrations available: Phosphate Buffered Saline: 1 L of 1X (sc-362182) Phosphate Buffered Saline: 100 ml of 10X (sc-362297) Phosphate Buffered Saline: 500 ml of 10X (sc-362298) Phosphate Buffered Saline: 1 L of 10X (sc-24946) Phosphate Buffered Saline: 5 L of 20X (sc-362299) Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300) Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301) Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302) Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)
1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792 2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548 3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994 4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324 5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070 6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.
• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 20 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
When diluted to a 1X concentration, the pH will be ~7.4.
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Phosphate Buffered Saline: 1 L of 20X
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Rated 5 out of
Various publications cite the use of PhosphateVarious publications cite the use of Phosphate Buffered Saline (PBS) as a generalized buffer in numerous applications. -SCBT Publication Review
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