Phosphate Buffered Saline: 1 L of 1X Ready to use 1X PBS solution frequently used as a wash and dilution buffer in biological research applications

Phosphate Buffered Saline: 1 L of 1X

Phosphate Buffered Saline: 1 L of 1X is rated 5.0 out of 5 by 1.
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Synonym: 1X PBS
Application: Ready to use 1X PBS solution frequently used as a wash and dilution buffer in biological research applications
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phosphate Buffered Saline: 1 L of 1X is a ready to use buffer solution commonly used in biological research. Phosphate-buffered saline (PBS) is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS buffer is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 100 ml of 10X (sc-362297)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 10X (sc-24946)
Phosphate Buffered Saline: 1 L of 20X (sc-362183)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)
Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)


References

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792
2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548
3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994
4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324
5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070
6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

Usage :
• Ready to use 1X PBS buffer is a solution with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. pH 7.4 ±0.2 (25°C).


• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).


• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
Storage :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Phosphate Buffered Saline: 1 L of 1X  Product Citations

See how others have used Phosphate Buffered Saline: 1 L of 1X. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 23946635  Joachim, SC. et al. 2013. Molecular vision. 19: 1804-14.

Citations 1 to 1 of 1 total

what is the usual concentration used for washing using 1X PBS?

Asked by: Shah23
Thank you for your question. Our ready to use 1X PBS buffer is a solution with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. pH 7.4 ±0.2 (25°C).
Answered by: Technical Support
Date published: 2019-01-03

What is recommended storage conditions of PBS?

Asked by: mmkstarr
Thank you for your question. PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use. This information is available on our website under the "Technical Information" tab, next to the "Description" tab, below the Ordering Information on our product page.
Answered by: Technical Support
Date published: 2017-02-15
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Rated 5 out of 5 by from Numerous publications cite the use of PBS Numerous publications cite the use of PBS in various biological research applications. -SCBT Publication Review
Date published: 2015-05-14
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