Phosphate Buffered Saline: 1 L of 10X is a concentrated buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Phosphate Buffered Saline is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption. Additional sizes, formats, and concentrations available: Phosphate Buffered Saline: 1 L of 1X (sc-362182) Phosphate Buffered Saline: 100 ml of 10X (sc-362297) Phosphate Buffered Saline: 500 ml of 10X (sc-362298) Phosphate Buffered Saline: 1 L of 20X (sc-362183) Phosphate Buffered Saline: 5 L of 20X (sc-362299) Phosphate Buffered Saline: powder for 100 ml of 10X (sc-362300) Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301) Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302) Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)
1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792 2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548 3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994 4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324 5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070 6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor. Vol. 3.
• Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 10 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
If diluted to [1X], pH will be ~7.4
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Phosphate Buffered Saline: 1 L of 10X
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Hi, my experiment needs 0.1M PBS solution, Is it possible to use this product to make 0.1M solution. If yes then please briefly explain me how?
Asked by: imran
Thank you for your inquiry. Our Phosphate Buffered Saline: 1 L of 10X makes 10 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate when diluted. It is provided with a 0.1M phosphate buffer concentration, prior to dilution.
Rated 5 out of
Cost effective and high quality productI purchased this reagent about three years ago and it still serves me well in making up 1x PBS stocks for general cell washing, or western blotting or IFF applications.
Date published: 2017-02-10
Rated 5 out of
EnunluEnunlu, I. et al. used product sc-24946 (Phosphate Buffered Saline: 1 L of 10X) with paraformaldehyde to create a fixation solution for mouse Neuro2A neuroblastoma cells. -SCBT Publication Review
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