Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.
Each vial contains 2.5 mM (-)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.
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See how others have used Phosphatase Inhibitor Cocktail A. Click on the entry to view the PubMed entry .
PMID: # 28957431 Wang, X. et al. 2017. PLoS Pathog. 13: e1006580.
PMID: # 27097660 Dickson, LM. et al. 2016. Am. J. Physiol. Regul. Integr. Comp. Physiol. 311: R79-88.
PMID: # 26706937 Seyithanoğlu, M. et al. 2016. Arch. Physiol. Biochem. 122: 94-102.
PMID: # 25623948 Bavamian, S. et al. 2015. Mol. Psychiatry. 20: 573-84.
PMID: # 26083323 Song, H. et al. 2015. Autophagy. 11: 1308-25.
PMID: # 25475437 Kaihara, KA. et al. 2015. Diabetes. 64: 1688-97.
PMID: # 26261332 Chamberlain, MD. et al. 2015. Proc. Natl. Acad. Sci. U.S.A. 112: 10673-8.
PMID: # 24723470 Gautherot, J. et al. 2014. Hepatology. 60: 610-21.
PMID: # 24463882 Lai, Y. et al. 2014. Human molecular genetics. 23: 3189-99.
PMID: # 25232250 Zmijewski, E. et al. 2014. World J. Gastroenterol. 20: 12161-70.
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