Phosphatase Inhibitor Cocktail A An inhibitor of L-isozymes of alkaline phosphatase and Ser/Thr protein phosphatases

Phosphatase Inhibitor Cocktail A

Phosphatase Inhibitor Cocktail A is rated 5.0 out of 5 by 3.
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Synonym: Phosphatase Inhibitor Cocktail I
Application: An inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.
Each vial contains 2.5 mM (-)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


References

1. Nishiwaki, S., et al. 1991. FEBS Lett. 279: 115-118. PMID: 1847341
2. Ilkovitch, D., et al. 2008. Cancer Res. 68: 2427-2435. PMID: 18381451
3. Wagner, M.W., et al. 2008. J. Biol. Chem. 283: 21382-21393. PMID: 18480061
4. Jain, M.K. 1982. Handbook of Enzyme Inhibitors. New York: John Wiley and Sons, Pg. 222, 334.

Usage :
• Recommended usage is 100-fold dilution.

• One ml of Phosphatase Inhibitor Cocktail A will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, cow liver, rabbit muscle, A431 or Jurkat cell extracts at a protein concentration of approximately 5 mg/ml.

• One ml of Phosphatase Inhibitor Cocktail A solution is used to prepare 100 ml of supernatant that contains a maximum of 500 mg of protein. Therefore, 1 ml of cocktail should be added per 500 mg of protein extracted from the tissue used, or 1 ml of cocktail solution per 100 ml of extraction buffer.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Phosphatase Inhibitor Cocktail A  Product Citations

See how others have used Phosphatase Inhibitor Cocktail A. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 27 total

PMID: # 28957431  Wang, X. et al. 2017. PLoS Pathog. 13: e1006580.

PMID: # 27097660  Dickson, LM. et al. 2016. Am. J. Physiol. Regul. Integr. Comp. Physiol. 311: R79-88.

PMID: # 26706937  Seyithanoğlu, M. et al. 2016. Arch. Physiol. Biochem. 122: 94-102.

PMID: # 25623948  Bavamian, S. et al. 2015. Mol. Psychiatry. 20: 573-84.

PMID: # 26083323  Song, H. et al. 2015. Autophagy. 11: 1308-25.

PMID: # 25475437  Kaihara, KA. et al. 2015. Diabetes. 64: 1688-97.

PMID: # 26261332  Chamberlain, MD. et al. 2015. Proc. Natl. Acad. Sci. U.S.A. 112: 10673-8.

PMID: # 24723470  Gautherot, J. et al. 2014. Hepatology. 60: 610-21.

PMID: # 24463882  Lai, Y. et al. 2014. Human molecular genetics. 23: 3189-99.

PMID: # 25232250  Zmijewski, E. et al. 2014. World J. Gastroenterol. 20: 12161-70.

Citations 1 to 10 of 27 total

Can this product ship to China?

Asked by: SCchem16
Thank you for your question. The compound, sc-45044, cannot ship overseas due to the presence of Microcystin-LR. A possible alternative which can be shipped overseas is sc-45065. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-24
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Rated 5 out of 5 by from Great phosphatase inhibitor Great phosphatase inhibitor! I add it on cell extracts 1:100 dilution, as its recommended, and works well!
Date published: 2015-10-13
Rated 5 out of 5 by from Used with frozen tissue sections and worked well Used with frozen tissue sections and worked well.
Date published: 2015-06-03
Rated 5 out of 5 by from By having this Phosphatase Inhibitor Cocktail By having this Phosphatase Inhibitor Cocktail A present in the cell lysate, de-phosphorylation is prevented by any phosphatase enzymes that may be present in the sample. This action is demonstrated by treating K-562 cell lysate (containing this inhibitor as part of the lysis buffer) with lambda protein phosphatase to see if the phosphorylation of the Cofilin 1 protein can be altered. By WB, we can see that this inhibitor cocktail does indeed prevent lambda protein phosphatase from de-phosphorylating p-Cofilin 1 protein. -SCBT QC
Date published: 2015-03-01
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