PGC-1 alpha Antibody (D-5)recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PGC-1α Antibody (D-5): sc-518025

PGC-1α Antibody (D-5) is rated 2.3 out of 5 by 4.
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Datasheets
    • PGC-1α Antibody (D-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping near the N-terminus of PGC-1α of human origin
    • recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PGC-1α (D-5): sc-518025.
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I am also interested in PGC1a sc-518028 antibody for use in Chromatin Immunoprecipitation and Immunofluorescence staining. Can you confirm that this works?

Asked by: Jeff20
Thank you for your question. We have not yet tested this antibody, nor are we aware of any publications citing use of this antibody for Chromatin Immunoprecipitation (ChIP). This antibody is recommended for immunofluorescent staining (IF) and we have published data available on our website now. There are also multiple product citations using this antibody for IF.
Answered by: Technical Support
Date published: 2019-02-10

I have two questions for the antibody.1: What is the exact molecule weight for WB, 130 or 100KD? 2. Can the antibody be used for flow analysis if I add Alexa Fluor® 488 antibody after incubation?

Asked by: Shaowei
Thank you for your question. The predicted molecular weight for full length PGC-1α1 is ~90 kDa, with the expected observed molecular weight ~115 kDa. This antibody has an N-terminal epitope and we have data detecting a smaller molecular weight isoform, NT-PGC-1α (NT(erminal)-PGC-1α, ~ 37 kDa. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-02-06

I'm interested in the new PGC-1α Antibody (D-5): sc-518025 for WB and IP in mouse liver lysates. However, in the datasheet I don't see a picture showing an endogenous full lenght PGC1a expression or a sucssefull IP, do you have such data? Thanks,

Asked by: Asael
Thanks for your question. The antibody is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA and has been established for these applications. There are two publications that have successfully used PGC-1α Antibody (D-5): sc-518025 in western blot: PMID 30246714 and PMID. So far we have not received any customer feedback for IP with this antibody. 30515824. Please contact technical service for support or to arrange for a free sample of this antibody to see if it is suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-02-10

你好,请问这个抗体可以申请免费的试用装吗?

Asked by: Alisen
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-02
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Rated 4 out of 5 by from Worked okay for western blot This antibody worked okay for western blot with 1:160 dilution. (100 ug protein, rat liver, using the recommended Santa Cruz secondary antibody). There was a faint signal (but detectable) and some background though.
Date published: 2019-07-26
Rated 1 out of 5 by from Not working Not working Don't waste your time. I bought this instead of discontined SC-13067.
Date published: 2019-06-20
Rated 1 out of 5 by from Doesn't work don't waste time! Dear All, we have tested PGC1-alfa AB from sc and none of them work so far, don't waste time!
Date published: 2018-08-24
Rated 3 out of 5 by from Antibody review This antibody is mostly localized in cytosol and worked in mouse brain tissue in IF at 1:500 dilution.
Date published: 2018-05-29
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