Peroxin 13 Antibody (D-5)recommended for detection of Peroxin 13 of mouse, rat and human origin by WB, IP, IF and ELISA

Peroxin 13 Antibody (D-5): sc-271477

Peroxin 13 Antibody (D-5) is rated 4.5 out of 5 by 2.
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Datasheets
    • Peroxin 13 Antibody (D-5) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 254-403 mapping at the C-terminus of Peroxin 13 of human origin
    • recommended for detection of Peroxin 13 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Peroxin 13 (D-5): sc-271477.
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What is the recommended starting dilution for Western blot for sc-271477: Peroxin 13 (D-5) monoclonal antibody?

Asked by: DefinitelyNotMatt
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from 效果可以 能曝出目的条带,效果可以,1:1000比例用也可以,回收了两次用没问题。就是没曝整条带,不知道有没有杂带。没有杂带的话就很好。
Date published: 2017-11-30
Rated 5 out of 5 by from Produced nice Western blot data of Peroxin Produced nice Western blot data of Peroxin 13 expression in K-562, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2014-03-24
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