Perforin 1 Antibody (F-1) recommended for detection of Perforin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Perforin 1 Antibody (F-1): sc-136994

Perforin 1 Antibody (F-1) is rated 5.0 out of 5 by 3.
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Datasheets
    • Perforin 1 Antibody (F-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 241-555 mapping at the C-terminus of Perforin 1 of human origin
    • recommended for detection of Perforin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Perforin 1 (F-1): sc-136994.
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I am using Perforin 1 (F-1): sc-136994 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked for human NK cell line I received a sample of Perforin (F-1) antibody and tested on NK92 human NK cells by western blot, it worked great!
Date published: 2017-02-14
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T and NK-92 whole cell lysates. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic staining Superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-24
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