Percoll PLUS A low endotoxin colloidal silica covalently coated with silane

Percoll® PLUS (CAS 65455-52-9)

Percoll PLUS | CAS 65455-52-9 is rated 5.0 out of 5 by 2.
  • y_2019, m_6, d_24, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_296039, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 86ms
  • REVIEWS, PRODUCT
Synonym: similar to: Centricoll™
Application: A low endotoxin colloidal silica covalently coated with silane
CAS Number: 65455-52-9
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Percoll® PLUS is a low endotoxin reagent composed of colloidal silica covalently coated with silane. It is used for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll® PLUS is non-toxic and re-sterilizable, even after adjustment to physiological ionic strength.


References

Vincent, R., Nadeau, D., Anal. Biochem., v. 141, 322-328 (1984).

Usage :
• Performed gradients can be stored for weeks without change in gradient shape, provided that the gradient remains unfrozen.

• Percoll® PLUS can be buffered within the pH range 5.5 to 10 without change in properties.

• Concentrated or diluted Percoll® PLUS can be resterilized by autoclaving for 30 min at 120°C.



Preparation of gradient material

Percoll® PLUS/Percoll® (sc-500790) are best used in balanced salt solutions, physiological saline, or 0.25 M sucrose. Cells can be separated on gradients in balanced salt solutions. Subcellular particles, however, tend to aggregate in the presence of salts and it is recommended that the separation of such particles be carried out in Percoll® PLUS diluted with sucrose (0.25 M final concentration).

The low osmolality of Percoll® PLUS permits this parameter to be controlled by the user without significant interference from the density medium itself. The addition of 9 parts (v/v) of Percoll® PLUS to 1 part (v/v) of either 1.5 M NaCl, 10x concentrated
cell culture medium, or 2.5 M sucrose will result in a solution adjusted to about 340 mOsm/kg H2O. Solutions of different osmotic pressure can be produced by adjusting the relative volumes of Percoll® PLUS and salt or sucrose solution. The final adjustment to the required osmolality can be carried out by the addition of salts or distilled water. When precise osmotic pressures are required, it is recommended that the osmolality of the solutions be measured in an osmometer.




Centrifugation with Percoll® PLUS

Percoll® PLUS will form self-generated gradients by
centrifugation at approximately 10,000 gav (in 0.15 M saline) or 25,000 gav (in 0.25 M sucrose) in fixed-angle rotor heads after 15 minutes. Cells or subcellular particles can be mixed with Percoll® PLUS prior to centrifugation and will band isopycnically, as the gradient is formed in situ. Although Percoll® PLUS is best used in angle-head rotors, banding of cells on pre-formed (continuous or discontinuous) gradients may be carried out at 400 gav for 20 to 30 minutes in swing-out rotors.



Removal of Percoll® PLUS after centrifugation

To remove the gradient medium from the biological material, perform one of the procedures outlined below.
• Cells can be recovered free from particles of Percoll® PLUS by dilution with physiological saline and centrifugation to collect the cells.
• Subcellular particles can be separated from Percoll® PLUS by the procedure described above. The size of the particles will determine the centrifugal force required to separate the particles from Percoll® PLUS.
• Gel filtration or ion exchange chromatography can also be used to separate biological material from Percoll® PLUS.
Formulation :
Colloidal solution of silica particles coated with silane
Appearance :
Suspension
Physical State :
Liquid
Solubility :
Soluble in water (partly miscible).
pH :
8.9–9.9
Storage :
Store at 4° C
Density :
1.1 g/mL at 25° C
Refractive Index :
n20D ~1.35
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
MDL Number :
MFCD00131830

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Percoll PLUS  Product Citations

See how others have used Percoll PLUS. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 26839332  Frank, EA. et al. 2016. Toxicologic pathology. 44: 211-25.

PMID: # 25580734  Zhou, W. et al. 2015. Nature cell biology. 17: 170-82.

PMID: # 26113472  Shintani-Ishida, K. et al. 2015. Int. J. Cardiol. 197: 26-32.

PMID: # 23503501  Shen, B. et al. 2013. Transplantation. 95: 663-70.

Citations 1 to 4 of 4 total

Please provide the density on each bottle, It is crucial for preparing solution of known density.

Asked by: CARLOS12312334542664
Thank you for your question. The density shouldn’t vary bottle to bottle. In the "Technical Information" tab on our website, we list the density as 1.1 mg/mL, with each vial containing 25 mL of suspension.
Answered by: Technical Support
Date published: 2019-01-15

Is this product ready to use, or does it need to be diluted?

Asked by: SCM4
This chemical, Percoll® PLUS (CAS 65455-52-9), is ready to use. It is a density gradient that will separate the differing cells or parts of a cell depending on what is desired to be separated and the conditions of centrifugation.
Answered by: Chemical Support 1
Date published: 2017-03-24
  • y_2019, m_6, d_24, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_296039, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 139ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Use this routinely in our labs and works well Use this routinely in our labs and works well Price benefit is very good choice
Date published: 2017-01-01
Rated 5 out of 5 by from Use this routinely in our labs and works well Use this routinely in our labs and works well.
Date published: 2015-08-13
  • y_2019, m_6, d_24, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_296039, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 36ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.