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Pepstatin A (CAS 26305-03-3)

Pepstatin A | CAS 26305-03-3 is rated 4.8 out of 5 by 4.

See product citations (16)

Synonym: Pepstatin; Isoval-Val-Val-Sta-Ala-Sta

Application: An inhibitor of HIV-1 and aspartic proteases

CAS Number: 26305-03-3

Purity: >90%

Molecular Weight: 685.89

Molecular Formula: C₃₄H₆₃N₅O₉

* Refer to Certificate of Analysis for lot specific data (including water content).

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Pepstatin A inhibits the aspartic proteases cathepsin D, pepsin, and renin. Additionally, it suppresses p53-dependent apoptosis in lymphoid cells as well as TNFα-induced apoptosis in U937 cells. Pepstatin A also efficiently inhibits HIV-1 protease and Fcγ receptor induction by IFN-γ.

References

1. Umezawa, H., et al. 1970. J. Antibiot. 23: 259-262. PMID: 4912600
2. Deiss, L.P., et al. 1996. EMBO J. 15: 3861-3870. PMID: 8670891
3. Wu, G.S., et al. 1998. Oncogene. 16: 2177-2183. PMID: 9619826
4. Athanassakis, I., et al. 2000. Cell. Immunol. 199: 81-88. PMID: 10698617
5. Farías, M.E., et al. 2000. FEMS Microbiol. Lett. 185: 263-266. PMID: 10754258

Usage :

Effective working concentration is a 1 μM solution, stable for at least 1 day at room temperature.

Physical State :

Solid

Sequence :

Isovaleryl-Val-Val-Sta-Ala-Sta

Solubility :

Soluble in 10% (v/v) acetic acid in methanol (9:1 methanol:acetic acid) (1 mg/ml), ethanol (1-2 mg/ml with heat up to 60° C), DMSO (5 mg/ml), methanol (1 mg/ml), and acetic acid. Insoluble in benzene, chloroform, water, 1 M NaOH, and ether.

Storage :

Store at -20° C

Melting Point :

233° C (dec.)

Boiling Point :

~997.6° C at 760 mmHg (Predicted)

Density :

~1.1 g/cm³ (Predicted)

Refractive Index :

n²⁰_D 1.50 (Predicted)

Optical Activity :

α20/D -90.0°±5°, c = 0.5 in MeOH

pK Values :

pK_a: 4.17 (Predicted)

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

RTECS :

SC6155000

PubChem CID :

5478883

Merck Index :

14: 7147

MDL Number :

MFCD00060740

EC Number :

247-600-0

Beilstein Registry :

2201362

SMILES :

C[C@@H](C(=O)N[C@@H](CC(C)C)[C@H](CC(=O)O)O)NC(=O)C[C@@H]([C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C)O

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Certificate of Analysis

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Pepstatin A Product Citations

See how others have used Pepstatin A. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 16 total

PMID: # 26730950 Markosyan, RM. et al. 2016. PLoS pathogens. 12: e1005373.

PMID: # 28105029 Welsby, I. et al. 2016. Front Immunol. 7: 663.

PMID: # 27297104 Okoro, EU. et al. 2016. Biochemical and biophysical research communications.

PMID: # 26935990 Wang, W. et al. 2016. Scientific reports. 6: 22550.

PMID: # 25042057 Kong, L. et al. 2015. Arch. Insect Biochem. Physiol. 88: 64-84.

PMID: # 26163003 Gorojod, RM. et al. 2015. Free Radic. Biol. Med. 87: 237-51.

PMID: # 24726834 Granato, M. et al. 2014. Biochimica et biophysica acta. 1843: 1348-55.

PMID: # 24880896 Valcheva, P. et al. 2014. Atherosclerosis. 235: 247-55.

PMID: # 25535899 Katona, BW. et al. 2014. Cancer Biol. Ther. 15: 1677-87.

PMID: # 24859454 Zhou, X. et al. 2014. Journal of hepatology. 61: 746-54.

Citations 1 to 10 of 16 total

Is this Pepstatin A  (CAS 26305-03-3) of microbial origin or synthetic ?

Asked by: Rahulgaur

Thank you for inquiring with us. Our Pepstatin A sc-45036 is synthetic in origin. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.

Answered by: Technical Service

Date published: 2017-07-04

Is this compound cell permeable?

Asked by: Jz28sail

Thank you for your question. sc-45036, Pepstatin A is not cell permeable. We recommend sc-222147, Pepstatin A Methyl Ester as a cell permeable alternative product (https://www.scbt.com/scbt/product/pepstatin-a-methyl-ester).

Answered by: Chemical Support 5

Date published: 2017-04-07

Rated 5 out of 5 by roxanagorojod from It works fine I employed this inhibitor to demonstrate the involvement of Cathepsin D in cell death. Pepstatin A was dissolved in DMSO. For assays, cells were incubated with Pepstatin A 10uM 1h prior to treatment.

Date published: 2017-01-26

Rated 5 out of 5 by NZ from This drug exerts its function pretty well This drug exerts its function pretty well in autophagy induction. Published data

Date published: 2015-11-06

Rated 4 out of 5 by KG from Works well for insect disections Works well for insect disections.

Date published: 2015-11-02

Rated 5 out of 5 by JL from Wang Wang, Y. et al. (PubMed 26258792) found that Pepstatin A, an inhibitor of aspartic proteases: cathepsin D, pepsin, and renin, relieved citreoviridin-induced apoptosis. -SCBT Publication Review

Date published: 2015-01-24

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Pepstatin A (CAS 26305-03-3)

QUICK LINKS

References

Download SDS (MSDS)

Certificate of Analysis

Pepstatin A Product Citations

Is this Pepstatin A  (CAS 26305-03-3) of microbial origin or synthetic ?

Is this compound cell permeable?

Pepstatin A (CAS 26305-03-3)

QUICK LINKS

References

Download SDS (MSDS)

Certificate of Analysis

Pepstatin A Product Citations

Is this Pepstatin A &nbsp;(CAS 26305-03-3) of microbial origin or synthetic ?

Is this compound cell permeable?

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Is this Pepstatin A (CAS 26305-03-3) of microbial origin or synthetic ?