PBEF Antibody (E-3)recommended for detection of PBEF of mouse, rat and human origin by WB, IP, IF and ELISA

PBEF Antibody (E-3): sc-393444

PBEF Antibody (E-3) is rated 5.0 out of 5 by 12.
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Datasheets
    • PBEF Antibody (E-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of PBEF of human origin
    • recommended for detection of PBEF of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PBEF (E-3): sc-393444.
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What is the recommended fixation method for immunofluorescence staining with PBEF (E-3): sc-393444 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Works well Probed at 1:500 overnight, detected strong bands at the right size
Date published: 2017-11-15
Rated 5 out of 5 by from Satisfied with the antibody PBEF antibody provides great staining in IF and IHC applications, binding is both strong and specific.The antibody could be performed under isothermal conditions, its specificity was good and the sensitivity.
Date published: 2017-10-09
Rated 5 out of 5 by from This antibody is very good. PBEF antibody works well for WB at the concentration of 1:200, with satisfied bands.
Date published: 2017-05-23
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-05-08
Rated 5 out of 5 by from Excellent antibody! I apply for the sample sack of this antibody,the band is special and clear.
Date published: 2017-03-29
Rated 5 out of 5 by from The Product is Good We tried it detect this sample from dilution 1:800. The researchers confirmed that PBEF Antibody accurately detects rat with high sensitivity and specificity. We were convinced the antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from WB and worked fine This gave very strong signal in our Western, we use dilution to 1:400 and it worked well.
Date published: 2017-02-12
Rated 5 out of 5 by from The use of this antibody signal is strong WB1:1000 diluted in rat cells with clear bands, strong signal.
Date published: 2017-02-05
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