Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The p53 tumor suppressor gene is altered in over 50% of human cancers. The p53 binding proteins 53BP1 and 53BP2 (Bbp) are tumor suppressors that bind to the site-specific central DNA-binding domain of wild type p53 in a conformation-dependent manner. Severe DNA damage can cause phosphorylation of p53 at position serine-46. This event triggers expression of p53AIP1 (apoptosis inducing protein), which contributes to subsequent events leading to programmed cell death. The protein PARC (p53 associated Parkin-like cytoplasmic protein) acts as a cytoplasmic anchor for p53 in unstressed cells, thereby regulating the localization and subsequent function of p53. The C-terminus of the PARC protein contains a RING-IBR-RING domain, which suggests it retains ubiquitin ligase activity, but PARC fails to promote degradation of p53. The gene encoding human PARC maps to chromosome 6p21.1.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using PARC (PO69): sc-53748 antibody for immunoprecipitation, how much antibody should I use?
Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
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