Paraformaldehyde solution 4% in PBSCommonly used to preserve tissues for routine histology

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Paraformaldehyde solution 4% in PBS | CAS 30525-89-4 is rated 4.9 out of 5 by 14.
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Synonym: 4% Formaldehyde in PBS
Application: Commonly used to preserve tissues for routine histology
CAS Number: 30525-89-4
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Paraformaldehyde solution 4% in PBS is commonly used to preserve tissues for routine histology in many labs. The solution should be clear, colorless, with no precipitate and the pH 7.0 - 7.6 (25°C). Supplied as a 4% Paraformaldehyde solution in 1X PBS.


References

1. Kim, TH. et al. 2016. Scientific reports. 6: 26687. PMID: 27220918

2. Chang, CY. et al. 2016. Nature neuroscience. 19: 111-6. PMID: 26642092

3. Zhou, H. et al. 2015. Stem cell reviews. 11: 652-65. PMID: 25951995

4. Paull, D. et al. 2015. Nature methods. 12: 885-92. PMID: 26237226

Usage :
We recommend solutions are used for no longer than 3 months from when they are made. Aliquot and freeze at -20°C for long-term storage.
Protect from light.
Formulation :
Contains methanol as a stabilizer.
Physical State :
Liquid
Solubility :
Soluble in water
pH :
(25°C): 7.0 - 7.6
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Paraformaldehyde solution 4% in PBS  Product Citations

See how others have used Paraformaldehyde solution 4% in PBS. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 95 total

PMID: # 31207939  Tsai, TH.|Lee, CH.|Cheng, CI.|Fang, YN.|Chung, SY.|Chen, SM.|Lin, CJ.|Wu, CJ.|Hang, CL.|Chen, WY.| et al. 2019. Cells. 8:

PMID: # 30898438  Leimbacher, PA.|Jones, SE.|Shorrocks, AK.|de Marco Zompit, M.|Day, M.|Blaauwendraad, J.|Bundschuh, D.|Bonham, S.|Fischer, R.|Fink, D.|Kessler, BM.|Oliver, AW.|Pearl, LH.|Blackford, AN.|Stucki, M.| et al. 2019. Mol. Cell.

PMID: # 31227505  Kazi, A.|Xiang, S.|Yang, H.|Chen, L.|Kennedy, P.|Ayaz, M.|Fletcher, S.|Cummings, C.|Lawrence, HR.|Beato, F.|Kang, Y.|Kim, MP.|Delitto, A.|Underwood, PW.|Fleming, JB.|Trevino, JG.|Hamilton, AD.|Sebti, SM.| et al. 2019. Clin. Cancer Res.

PMID: # 31235253  Guo, Y.|Gilbert-Honick, J.|Somers, SM.|Mao, HQ.|Grayson, WL.| et al. 2019. Biochem. Biophys. Res. Commun. 516: 558-564.

PMID: # 30852603  Akamandisa, MP.|Nie, K.|Nahta, R.|Hambardzumyan, D.|Castellino, RC.| et al. 2019. Neuro-oncology. 21: 786-799.

PMID: # 31327667  Li, Y. et al. 2019. Cell Metab.

PMID: # 30755440  Viitala, M.|Virtakoivu, R.|Tadayon, S.|Rannikko, J.|Jalkanen, S.|Hollmén, M.| et al. 2019. Clin. Cancer Res. 25: 3289-3303.

PMID: # 30822688  Barazzuol, L.|Hopkins, SR.|Ju, L.|Jeggo, PA.| et al. 2019. DNA Repair (Amst.). 76: 70-75.

PMID: # 30792658  Lee, C.|Liao, J.|Chen, S.|Yen, C.|Lee, Y.|Huang, S.|Huang, S.|Lin, C.|Chang, VH.| et al. 2019. Front Pharmacol. 10: 91.

PMID: # 31189704  Liu, X.|Matrenec, R.|Gack, MU.|He, B.| et al. 2019. J. Virol. 93:

Citations 1 to 10 of 95 total

what is the expiration of the PFA solution if kept at 4 C.

Asked by: Uriw
Thank you for your question. We provide a warranty of one year on the viability of our products given proper storage conditions. To find out the lot-specific expiry date please contact our technical service or your local distributor for expiration information for the specific lot number of your purchased product.
Answered by: Tech Service
Date published: 2019-08-20

Is it suitable for cell fixation during intracellular staining for flow cytometry?

Asked by: Shani
Thank you for your question. We recommend using our FCM Fixation Buffer: sc-3622 for fixing an permeabilizing cells for intracellular staining.
Answered by: Technical Support
Date published: 2019-08-17

Is this product in stock and ready to be shipped out once it is ordered?

Asked by: Curious123
Thank you for your question. Please contact our Customer Care Department for current product availability.
Answered by: Technical Support
Date published: 2019-07-23

What percent methanol does this product contain? Is this product suitable for fixation for downstream detection of endogenous EGFP-tagged protein?

Asked by: Cindy Ohio
Thank you for your question. This product, sc-281692, is a Paraformaldehyde solution 4% in PBS that contains 2.5% methanol. It is used for routine histology fixation, which includes immunofluorescence applications. We can not speak for a specific tagged sample as it is necessary to optimize fixation conditions for every sample, but would expect it to be perfectly fine for that use.
Answered by: Tech Service
Date published: 2019-07-08

would need 5liters for june 6, is this stock ? please confirm price.

Asked by: cobra ben
Thank you for your question. Please contact our Customer Care Department for current product availability, and to request a formal quotation. Current pricing is available on our website.
Answered by: Technical Support
Date published: 2019-01-26

Hola buenas tardes . Tengo una duda acerca del producto paraformaldehyde 4 % in PBS, (SC-281692). ¿Tiene fecha de caducidad? No la encuentro y no sé durante cuánto tiempo se puede usar. El número de lote es D1614. Muchas gracias

Asked by: AnaFernándezCarrascal
Gracias por su pregunta. Por favor contacte Servicio Tecnico para obtener mas informacion. Thank you for your question. Please contact Technical Service for more information.
Answered by: Technical Support
Date published: 2018-12-06

J'aimerai me procurer la fiche Safety du PFA 4% in PBS (sc-281692), merci de me l'adresser. A long terme > à 2 mois, comment conserver ce produit (congélation ? et à quelle température ? -20°C ou -80°C. Bien cordialement.

Asked by: NATHASHA
Merci pour votre question. La fiche de sécurité du produit sc-281692 est téléchargeable en ligne, directement sur la page web du produit. Pour une conservation sur le long terme, nous recommandons d’aliquoter et de conserver ce produit à -20°C. Thank you for your question. The MSDS of sc-28169 is available online, directly on the product webpage. Regarding the storage of sc-281692, we recommend to aliquot and store the product at -20°C for a long-term storage.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Can I divide the PFA solution in smaller amounts (let's say 25 ml) and put them in a -20° C freezer?

Asked by: miguel b
Thank you for your question. Yes, we recommend to aliquot and freeze at -20°C for long-term storage.
Answered by: Tech Service
Date published: 2019-02-15
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Rated 5 out of 5 by from great features! My lab has been ordering this and we are very satisfied with it
Date published: 2019-07-02
Rated 5 out of 5 by from Good PFA solution We bought this reagent one month ago. High quality and easy to store and use!
Date published: 2018-04-11
Rated 4 out of 5 by from works for fixation of cells It works for fixation of human cells in culture for cell imaging experiments.
Date published: 2018-02-20
Rated 5 out of 5 by from 4% Paraformaldehyde solution works THe delivery was on time. And the solution worked well. Saved a lot of time to prepare for it.
Date published: 2017-07-05
Rated 5 out of 5 by from Great!!! Ready to use. You do not need to dilute. Nice tissue fixation. Works very well for us.
Date published: 2016-12-30
Rated 5 out of 5 by from Specific and Sensitive Wonderful results using this antibody for IP/WB and IF with human samples.
Date published: 2016-10-28
Rated 5 out of 5 by from This solution is very good This solution is very good, has high quality and will order again.
Date published: 2015-09-15
Rated 5 out of 5 by from We use it routinely in our lab for IHC and We use it routinely in our lab for IHC and works well.
Date published: 2015-06-01
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