Paraformaldehyde solution 4% in PBS Commonly used to preserve tissues for routine histology

Paraformaldehyde solution 4% in PBS (CAS 30525-89-4)

Paraformaldehyde solution 4% in PBS | CAS 30525-89-4 is rated 4.9 out of 5 by 13.
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Synonym: 4% Formaldehyde in PBS
Application: Commonly used to preserve tissues for routine histology
CAS Number: 30525-89-4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Commonly used to preserve tissues for routine histology in many labs. The solution should be clear, colorless, with no precipitate and the pH 7.0 - 7.6 (25°C). Supplied as a 4% Paraformaldehyde solution in 1X PBS.

Usage :
Aliquot and freeze at -20°C for long-term storage.
Formulation :
Contains methanol as a stabilizer.
Physical State :
Liquid
Solubility :
Soluble in water
pH :
(25°C): 7.0 - 7.6
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Paraformaldehyde solution 4% in PBS  Product Citations

See how others have used Paraformaldehyde solution 4% in PBS. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 63 total

PMID: # 28779497  Proskorovski-Ohayon, R. et al. 2017. Hum. Mutat. 38: 1671-1683.

PMID: # 28024296  Matsumoto, A. et al. 2017. Nature. 541: 228-232.

PMID: # 28871090  Sparrer, KMJ. et al. 2017. Nat Microbiol. 2: 1543-1557.

PMID: # 28112381  Huang, G. et al. 2017. Int. J. Mol. Med. 39: 559-568.

PMID: # 28936468  Petschnigg, J. et al. 2017. Bio Protoc. 7:

PMID: # 27110779  Douvaras, P. et al. 2016. Int J Mol Sci. 17:

PMID: # 27220918  Kim, TH. et al. 2016. Scientific reports. 6: 26687.

PMID: # 27125279  Cappato, S. et al. 2016. Dis Model Mech.

PMID: # 26825118  Kamaruzzaman, NF. et al. 2016. The Journal of antimicrobial chemotherapy. 71: 1252-9.

PMID: # 26738794  Emin, M. et al. 2016. Science translational medicine. 8: 320ra1.

Citations 1 to 10 of 63 total

would need 5liters for june 6, is this stock ? please confirm price.

Asked by: cobra ben
Thank you for your question. Please contact our Customer Care Department for current product availability, and to request a formal quotation. Current pricing is available on our website.
Answered by: Technical Support
Date published: 2019-01-26

Hola buenas tardes . Tengo una duda acerca del producto paraformaldehyde 4 % in PBS, (SC-281692). ¿Tiene fecha de caducidad? No la encuentro y no sé durante cuánto tiempo se puede usar. El número de lote es D1614. Muchas gracias

Asked by: AnaFernándezCarrascal
Gracias por su pregunta. Por favor contacte Servicio Tecnico para obtener mas informacion. Thank you for your question. Please contact Technical Service for more information.
Answered by: Technical Support
Date published: 2018-12-06

J'aimerai me procurer la fiche Safety du PFA 4% in PBS (sc-281692), merci de me l'adresser. A long terme > à 2 mois, comment conserver ce produit (congélation ? et à quelle température ? -20°C ou -80°C. Bien cordialement.

Asked by: NATHASHA
Merci pour votre question. La fiche de sécurité du produit sc-281692 est téléchargeable en ligne, directement sur la page web du produit. Pour une conservation sur le long terme, nous recommandons d’aliquoter et de conserver ce produit à -20°C. Thank you for your question. The MSDS of sc-28169 is available online, directly on the product webpage. Regarding the storage of sc-281692, we recommend to aliquot and store the product at -20°C for a long-term storage.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Can I divide the PFA solution in smaller amounts (let's say 25 ml) and put them in a -20° C freezer?

Asked by: miguel b
Thank you for your question. Yes, we recommend to aliquot and freeze at -20°C for long-term storage.
Answered by: Tech Service
Date published: 2019-02-15

What's the total volume of this product?

Asked by: NPIKS
Thank you for your question. We provide this Paraformaldehyde solution 4% in PBS: sc-281692, in a total volume of 1 L.
Answered by: Technical Support
Date published: 2018-06-21

what is meant for PBS

Asked by: prabath
Thank you for your question. The PBS means that it is supplied in PBS (Phosphate Buffered Saline). If you have any further questions, please contact Technical Service for more information.
Answered by: Tech Service
Date published: 2017-11-06

What ratio of solution to tissue should be used (preferably stated as volume of solution per g of tissue)?

Asked by: AFDo
Thank you for your question. We have not determined a specific ratio of solution to tissue. We recommend using sufficient volume of Paraformaldehyde solution 4% in PBS (sc-281692) to completely cover and saturate the prepared tissue sections on your slides. Below is a link to our recommended protocol for immunoperoxidase staining of cells or tissues. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-11-06

Is this solution made with DEPC treated PBS? Is this solution RNase free? Is it possible to autoclave this solution ? 

Asked by: SCM4
This chemical, Paraformaldehyde solution 4% in PBS, is not made with DEPC treated PBS. We can make a batch with DEPC treated water as a special order, but our lab cannot verify that it is RNase-free. No, PFA solutions cannot be autoclaved.
Answered by: Chemical Support 1
Date published: 2017-11-06
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Rated 5 out of 5 by from Good PFA solution We bought this reagent one month ago. High quality and easy to store and use!
Date published: 2018-04-11
Rated 4 out of 5 by from works for fixation of cells It works for fixation of human cells in culture for cell imaging experiments.
Date published: 2018-02-20
Rated 5 out of 5 by from 4% Paraformaldehyde solution works THe delivery was on time. And the solution worked well. Saved a lot of time to prepare for it.
Date published: 2017-07-05
Rated 5 out of 5 by from Great!!! Ready to use. You do not need to dilute. Nice tissue fixation. Works very well for us.
Date published: 2016-12-30
Rated 5 out of 5 by from Specific and Sensitive Wonderful results using this antibody for IP/WB and IF with human samples.
Date published: 2016-10-28
Rated 5 out of 5 by from This solution is very good This solution is very good, has high quality and will order again.
Date published: 2015-09-15
Rated 5 out of 5 by from We use it routinely in our lab for IHC and We use it routinely in our lab for IHC and works well.
Date published: 2015-06-01
Rated 5 out of 5 by from Works well with perfusion Works well with perfusion, orders routinely.
Date published: 2015-05-05
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