PAR-2 Antibody (SAM11) recommended for detection of PAR-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

PAR-2 Antibody (SAM11): sc-13504

PAR-2 Antibody (SAM11) is rated 4.9 out of 5 by 10.
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Datasheets
    • PAR-2 Antibody (SAM11) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • epitope mapping within amino acids 37-50 of PAR-2 of human origin
    • recommended for detection of PAR-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • also provided azide-free for neutralization studies, sc-13504 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of PAR-2 (SAM11): sc-13504.
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I would like to use the PAR-2 Antibody (SAM11): sc-13504 to double stain my tissue samples with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Yes, this is definitely possible. In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the PAR-2 Antibody (SAM11): sc-13504 is available conjugated to HRP (sc-13504 HRP), phycoerythrin (sc-13504 PE), fluoroscein (sc-13504 FITC), Alexa Fluor® 488 (sc-13504 AF488), Alexa Fluor® 647 (sc-13504 AF647), or Alexa Fluor® 405 (sc-13504 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from robust band This antibody raised against the PAR-2,I tried diffenct conditions, 1:600 recommend for WB,band is very roubust.
Date published: 2017-06-24
Rated 5 out of 5 by from A good antibody Used this antibody in WB at 1:200 dilution, clear and strong.
Date published: 2017-04-05
Rated 5 out of 5 by from strong staining. Excellent results by IF, on mouse skeletal muscle 1:200,strong staining.
Date published: 2017-02-26
Rated 5 out of 5 by from good result This has worked well in IHC, 1:1000 dilution used for human stomach tissue.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent! This is an overall great antibody. Lots of publications, and works well in multiple applications. Solid choice.
Date published: 2016-12-27
Rated 5 out of 5 by from The AF488 conjugate of the antibody works The AF488 conjugate of the antibody works great in flow cytometry.
Date published: 2015-10-28
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-05-20
Rated 4 out of 5 by from This antibody has worked well to detect PAR This antibody has worked well to detect PAR-2 in human samples.
Date published: 2015-04-03
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