pan-Cytokeratin Antibody (AE1/AE3) recommended for detection of most acidic (type I) and basic (type II) Cytokeratins of mouse, rat, human, bovine and rabbit origin by WB, IP, IF and IHC(P)

pan-Cytokeratin Antibody (AE1/AE3): sc-81714

pan-Cytokeratin Antibody (AE1/AE3) is rated 5.0 out of 5 by 9.
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Datasheets
    • pan-Cytokeratin Antibody (AE1/AE3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against epidermal keratins of human origin
    • recommended for detection of most acidic (type I) and basic (type II) Cytokeratins of mouse, rat, human, bovine and rabbit origin by WB, IP, IF and IHC(P)
    • available conjugated to HRP for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of pan-Cytokeratin (AE1/AE3): sc-81714.
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Can I use this antibody in IF against primary cells collected from a mouse to detect pan CK?

Asked by: aroberts
Thank you for your question. Yes, this mouse monoclonal antibody, pan-Cytokeratin (AE1/AE3): sc-81714, is recommended for IF in mouse cells.
Answered by: Technical Support
Date published: 2017-11-15

I no longer have the goat anti-mouse secondary I was using. What secondary reagent do you recommend?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from strong signal in western blot tested this in HCC cell lines. works great by western blot
Date published: 2018-04-03
Rated 5 out of 5 by from Works with Human skin We got this as a sample to test with our tissues. We used human skin at a concentration of 1:250 and found it stained very well.
Date published: 2017-09-22
Rated 5 out of 5 by from Very good antibody I used this antibody on formalin-fixed paraffin embedded human squamous carcinoma tissues at a 1:30 dilution for 15 minutes. Staining was vibrant and crisp.
Date published: 2017-08-10
Rated 5 out of 5 by from all good The study found that cytokeratin is a useful marker of tissue differentiation, which can be directly applied to the characterization of malignant tumors. For example, cytokeratin 10 and 13 are highly expressed in subgroups of squamous cell carcinomas, whereas cytokeratin 18 is expressed in most adenocarcinomas and basal cell carcinomas
Date published: 2017-02-08
Rated 5 out of 5 by from Great product Very nice data in several applications including if ,ihc and wb
Date published: 2017-01-02
Rated 5 out of 5 by from Great protein recognition! SC-81714 is a great choice for cytokeratin detection. Its ability to recognize both acidic and basic subfamily members results in fantastic staining. This antibody has been featured in more than 15 publications.
Date published: 2016-12-16
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human skin. -SCBT QC
Date published: 2015-01-20
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