P2X3 Antibody (B-5) recommended for detection of P2X3 of mouse, rat and human origin by WB, IP, IF and ELISA

P2X3 Antibody (B-5): sc-390572

P2X3 Antibody (B-5) is rated 5.0 out of 5 by 3.
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Datasheets
    • P2X3 Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 338-397 mapping at the C-terminus of P2X3 of human origin
    • recommended for detection of P2X3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of P2X3 (B-5): sc-390572.
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We tested the P2X3 (B-5, sc-390572) mouse monoclonal antibody in Western blots with mouse dorsal root ganglion tissue. Unfortunately there is only unpsecific band from secondary antibody (anti-mouse-IgG1 680, Licor). We didn´t detect band aroud 43 kDa.

Asked by: Ruirui Lu
Thank you for your question. Please contact our Technical Service Department and we would be happy to help you troubleshoot the antibody. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-11-02

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-390572: P2X3 (B-5) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from Nice Western Blot data of P2X3 expression in RT Nice Western Blot data of P2X3 expression in RT-4 whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Produced positive Western Blot data of P2X3 Produced positive Western Blot data of P2X3 expression in T24 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2014-08-18
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