p16-ARC Antibody (C-3) recommended for detection of p16-ARC of mouse, rat and human origin by WB, IP, IF and ELISA

p16-ARC Antibody (C-3): sc-166760

p16-ARC Antibody (C-3) is rated 4.9 out of 5 by 8.
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Datasheets
    • p16-ARC Antibody (C-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-151 representing full length p16-ARC of human origin
    • recommended for detection of p16-ARC of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p16-ARC (C-3): sc-166760.
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What is the recommended fixation method for immunofluorescence staining with p16-ARC (C-3): sc-166760 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24

Do you know if your p16 antibody 166760 has been used in flow cytometry?

Asked by: Jolly
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Rated 5 out of 5 by from the antibody is well the antibody is well,i used it to do IHC,dilution in 1:50
Date published: 2017-12-28
Rated 5 out of 5 by from This p16 antibody works well! This p16 antibody works well without nonspecific bands.
Date published: 2017-10-27
Rated 5 out of 5 by from Highly recommended It has signals in the tissues of mice, and it's very clear, play an important role in cancer cell lysates, It's worth recommending and buying.
Date published: 2017-10-18
Rated 5 out of 5 by from magnificent This antibody works well when we use 1:2000 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-09-20
Rated 4 out of 5 by from sometimes work I use tissue to do Western blot. and sometimes it's work and sometimes doesn't work.
Date published: 2017-08-19
Rated 5 out of 5 by from very good Strong band with clean background in western blot with HL-60 whole cell lysate (1/500)
Date published: 2016-12-17
Rated 5 out of 5 by from IF publishable data with rat brain IF publishable data with rat brain - SCBT Publication Review
Date published: 2015-02-03
Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmic Produced exceptional immunofluorescence cytoplasmic and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2013-09-30
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