p15/p16 Antibody (C-7) recommended for detection of p15 and p16 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p15/p16 Antibody (C-7): sc-377412

p15/p16 Antibody (C-7) is rated 4.2 out of 5 by 20.
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Datasheets
    • p15/p16 Antibody (C-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 96-138 mapping at the C-terminus of p15 of human origin
    • recommended for detection of p15 and p16 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of p15/p16 (C-7): sc-377412.
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I'm getting a band at roughly 24kDa for whole mouse bone marrow lysate with this antibody. I'm not getting any bands near 15 or 16kDa. Do you have any recommendations? I've run several westerns with the same result

Asked by: yim123
Thank you for your question. We would recommend including a positive control, such as 3T3-L1 whole cell lysate (sc-2243) for comparison and to help you troubleshoot the antibody. We would recommend using the primary antibody at a starting dilution of 1:200, and loading 40-60 µg of protein. A link to our complete recommended protocol for Western blotting with this antibody is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Please contact our Technical Service Department and we are happy to help you troubleshoot the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-02

p15/p16 (C-7): sc-377412의 무료10 µg 샘플을 신청합니다.

Asked by: junguee
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다.sc-377412 무료10 µg 샘플이 있습니다. 신청하려면 우리 회사 한국에 있는 distributor에게 연락해 주십시오.아래는 연락처입니다. 다른 문의사항은 언제든지 메일로 기술지원팀에게 보내주시기 바랍니다 We have sc-377412 free sample for test.You can contact your local distributor to request SCBT antibody sample.Below is the address of our distributor in Korea.Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions. Your local distributor(s): Essence Medical Inc. 417 Leaders B/D, Yatapdong 342-1 Bundanggu Seongnam Gyeonggi-do Korea Tel. 82-31-707-1544 FAX: 82-31-707-1999 Email: info@essencemedical.com Website: http://www.essencemedical.com Koram Biotech Corporation 6F, Koram Venture Building 907-1 Daechi-Dong Kang Nam-Ku Seoul 135-080 Korea Tel. 82 (2) 556-0311 FAX: 82 (2) 556-0828 Email: koram@korambiotech.com Website: http://www.korambiotech.com
Answered by: Technical Service 11
Date published: 2017-08-17

Can this antibody be used to detect p16 in canine samples?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service
Date published: 2016-12-22
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Rated 2 out of 5 by from Didn't work Unfortunately we couldn't make it to work on our rat cell line (western blot).
Date published: 2018-08-01
Rated 3 out of 5 by from Works well for WB but not for IF WB works at a dilution of 1:250 on neonatal mouse lung lysates. Protein loaded 30ug
Date published: 2017-12-18
Rated 1 out of 5 by from P16 Thumbs down Didn't work for me unfortunately even at 1:100 in A2780 and SKOV-3 Ovarian Cancer cell lysates, despite success using GAPDH
Date published: 2017-11-29
Rated 5 out of 5 by from Works well for WB but not for IF Can be used at a dilution of 1:200 for WB on mouse lung lysates with 30ug protein loaded
Date published: 2017-11-08
Rated 5 out of 5 by from the result satisfied The researchers confirmed that knowledge of the sensitivity and specificity of p15/p16 Antibody leads to their more rational selection.
Date published: 2017-10-21
Rated 4 out of 5 by from Good antibody for IB This antibody works well in the immunoblotting assay. Meanwhile, unspecific bands could also be detected.
Date published: 2017-10-08
Rated 5 out of 5 by from does not work 1:500 diluted for wb of HL-60 and MOLM13 protein,there is no band
Date published: 2017-09-19
Rated 5 out of 5 by from 在人细胞上好用,鼠细胞还需测试 申请的试用装,室温情况下运输了几天,WB结果显示在人的细胞上好用。小鼠细胞还没检测,待检测后再做评论。
Date published: 2017-09-18
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