p-Tyr Antibody (PY99) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
raised against p-Tyr
recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
available conjugated to biotin for WB, IHC(P) and ELISA; and to Alexa Fluor® 405 for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Tyr (PY99): sc-7020.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The critical involvement of protein tyrosine kinases in signal transduction pathways is well established. These kinases can be divided into two major groups, including the receptor tyrosine kinases and the non-receptor type kinases, of which the Src kinases are the prototypical members. Src kinases are generally associated with the internal portion of the plasma membrane and may function as signal transducers in association with surface receptors that lack an intracellular catalytic domain. The second major group of tyrosine kinases are the receptor tyrosine kinases. More than fifty members of this group of receptors, belonging to fourteen families, have been identified to date. Ligand-induced tyrosine phosphorylation of such receptors induces receptor dimerization and subsequent autophosphorylation of specific individual phosphotyrosine residues located within their cytoplasmic domains, which serve as binding sites that interact with specific cytoplasmic molecules. Monoclonal antibodies to phosphotyrosine are valuable for the characterization and purification of proteins containing phosphotyrosyl residues, and are used extensively for these purposes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I want to measure insulin sensitivity and several tyrosine domains are phosphorylated in response to insulin. For example in both insulin receptors and in IRS. Does the antibody detetct all p-tyr, and I differentiate between them depending on weight?
Asked by: Ahmed_hassan
Thank you for your question. Yes, you are correct, this mouse monoclonal antibody, p-Tyr (PY99): sc-7020, specifically detects phosphotyrosine-containing proteins (and does not cross-reactive with phosphoserine or phosphothreonine-containing proteins). If you are using the antibody for Western blotting, then the molecular weight you detect in your samples is representative of different phosphorylated proteins. You would need to do further testing to identify which specific proteins are phosphorylated in your samples.
Answered by: Technical Support
Date published: 2017-10-09
What secondary reagent should I use with p-Tyr Antibody (PY99) for Western blot?
Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
Answered by: Tech Service 8
Date published: 2016-12-15
Is it possible to use this p-Tyr (PY99) mouse monoclonal antibody for immunohistochemistry in mouse tissue?
Asked by: Trav11
Yes, it is possible. If working with mouse tissue, we recommend using our directly conjugated mouse monoclonal primary antibodies. The p-Tyr Antibody (PY99): sc-7020 is currently available conjugated to phycoerythrin (sc-7020 PE), fluorescein (sc-7020 FITC), Alexa Fluor® 488 (sc-7020 AF488), Alexa Fluor® 647 (sc-7020 AF647), Alexa Fluor® 405 (sc-7020 AF405), biotin (sc-7020 B) or alkaline phosphatase (sc-7020 AP).
Answered by: Tech Service 8
Date published: 2016-12-14
Is there any species specificity for this antibody?
Or is there any cross-reactivity?
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