p-Tyr Antibody (PY20)
  • p-Tyr Antibody (PY20) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
  • raised against p-Tyr
  • recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM
  • available conjugated to agarose (sc-508 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-508 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-508 PE), fluorescein (sc-508 FITC), Alexa Fluor® 488 (sc-508 AF488) or Alexa Fluor® 647 (sc-508 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 405 (sc-508 AF405, 100 µg/2 ml) for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.

p-Tyr Antibody (PY20): sc-508

p-Tyr Antibody (PY20) is rated 4.9 out of 5 by 14.
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Datasheets
    • p-Tyr Antibody (PY20) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against p-Tyr
    • recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose (sc-508 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-508 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-508 PE), fluorescein (sc-508 FITC), Alexa Fluor® 488 (sc-508 AF488) or Alexa Fluor® 647 (sc-508 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 405 (sc-508 AF405, 100 µg/2 ml) for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
p-Tyr Antibody (PY20) sc-508 200 µg/ml $279.00
p-Tyr Antibody (PY20) AC sc-508 AC 500 µg/ml, 25% ag $366.00
p-Tyr Antibody (PY20) Alexa Fluor® 405 sc-508 AF405 100 µg/2 ml $314.00
p-Tyr Antibody (PY20) Alexa Fluor® 488 sc-508 AF488 200 µg/ml $314.00
p-Tyr Antibody (PY20) Alexa Fluor® 647 sc-508 AF647 200 µg/ml $314.00
p-Tyr Antibody (PY20) FITC sc-508 FITC 200 µg/ml $292.00
p-Tyr Antibody (PY20) HRP sc-508 HRP 200 µg/ml $279.00
p-Tyr Antibody (PY20) PE sc-508 PE 200 µg/ml $303.00

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p-Tyr Antibody (PY20) Product Citations

See how others have used p-Tyr Antibody (PY20). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 389 total

PMID: # 26883159
He, Y. et al. 2016. Interaction of EGFR to δ-catenin leads to δ-catenin phosphorylation and enhances EGFR signaling. Sci Rep. 6: 21207.

PMID: # 27448998
Richter, B. et al. 2016. Klotho modulates FGF23-mediated NO synthesis and oxidative stress in human coronary artery endothelial cells. Pflugers Arch.. 468: 1621-35.

PMID: # 27494324
Stucky, A. et al. 2016. Prenatal Cocaine Exposure Upregulates BDNF-TrkB Signaling. PLoS ONE. 11: e0160585.

PMID: # 27666821
Boo, HJ. et al. 2016. The tobacco-specific carcinogen-operated calcium channel promotes lung tumorigenesis via IGF2 exocytosis in lung epithelial cells. Nat Commun. 7: 12961.

PMID: # 26052252
Moazzam, M. et al. 2015. Knockdown of WAVE3 impairs HGF induced migration and invasion of prostate cancer cells. Cancer cell international. 15: 51.

PMID: # 26079946
Ma, S. et al. 2015. Tyrosine dephosphorylation enhances the therapeutic target activity of epidermal growth factor receptor (EGFR) by disrupting its interaction with estrogen receptor (ER). Oncotarget. 6: 13320-33.

PMID: # 26280128
Oliveira, V. et al. 2015. Diets Containing α-Linolenic (ω3) or Oleic (ω9) Fatty Acids Rescues Obese Mice From Insulin Resistance. Endocrinology. 156: 4033-46.

PMID: # 25482129
Motiwala, T. et al. 2015. PTPROt-mediated regulation of p53/Foxm1 suppresses leukemic phenotype in a CLL mouse model. Leukemia. 29: 1350-9.

PMID: # 24523872
Miah, S. et al. 2014. BRK Targets Dok1 for Ubiquitin-Mediated Proteasomal Degradation to Promote Cell Proliferation and Migration. PloS one. 9: e87684.

PMID: # 25025568
Bartolome, R.A. et al. 2014. A Blk–p190RhoGAP signaling module downstream of activated Gα13 functionally opposes CXCL12-stimulated RhoA activation and cell invasion Cell Signal. . 26: 2551-2561.

Citations 1 to 10 of 389 total

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Answered by: Tech Service 6
Date published: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
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Rated 5 out of 5 by from good result antibody It is an excellent antibody. gives a single band in my hand.
Date published: 2017-05-27
Rated 5 out of 5 by from antibody for WB I use the dilution 1:800, get very clean bands.I like
Date published: 2017-05-07
Rated 5 out of 5 by from High Quality Great antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Date published: 2016-12-20
Rated 4 out of 5 by from Good antibody but not as sensitive as before The antibody works good with Western blotting and immunofluorescence. However, the current lot of pY20 detects fewer tyrosine phosphorylated bands in whole cell lysates than previous lots of the same antibody. Perhaps increasing the working conc. of the antibody may help. But still, a very cheap alternative to 4G10.
Date published: 2016-10-24
Rated 5 out of 5 by from Greatly works in immunostaining and shows Greatly works in immunostaining and shows clear membrane phosphorylation patterns
Date published: 2016-07-29
Rated 5 out of 5 by from Works as expected Works as expected
Date published: 2015-11-06
Rated 5 out of 5 by from It works well It works well.
Date published: 2015-09-30
Rated 5 out of 5 by from No comment No comment
Date published: 2015-09-23
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