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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The critical involvement of protein tyrosine kinases in signal transduction pathways is well established. These kinases can be divided into two major groups, including the receptor tyrosine kinases and the non-receptor type kinases, of which the Src kinases are the prototypical members. Src kinases are generally associated with the internal portion of the plasma membrane and may function as signal transducers in association with surface receptors that lack an intracellular catalytic domain. The second major group of tyrosine kinases are the receptor tyrosine kinases. More than fifty members of this group of receptors, belonging to fourteen families, have been identified to date. Ligand-induced tyrosine phosphorylation of such receptors induces receptor dimerization and subsequent autophosphorylation of specific individual phosphotyrosine residues located within their cytoplasmic domains, which serve as binding sites that interact with specific cytoplasmic molecules. Monoclonal antibodies to phosphotyrosine are valuable for the characterization and purification of proteins containing phosphotyrosyl residues, and are used extensively for these purposes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PMID: # 26616860 Debruyne, DN. et al. 2016. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT. Oncogene. 35: 3681-91.
PMID: # 22235846 Nadal-Casellas A. et al. 2012. Sex-dependent differences in rat hepatic lipid accumulation and insulin sensitivity in response to diet-induced obesity. Biochem Cell Biol. . 90(2): 164-172.
I am using p-Tyr (2C8): sc-81529 antibody for immunoprecipitation, how much antibody should I use?
Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
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