p-Ser Antibody (16B4)
  • p-Ser Antibody (16B4) is a mouse monoclonal IgM provided at 50 µg/0.5 ml
  • raised against phosphoserine
  • recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP
  • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of p-Ser (16B4): sc-81514.

p-Ser Antibody (16B4): sc-81514

p-Ser Antibody (16B4) is rated 4.3 out of 5 by 15.
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Datasheets
    • p-Ser Antibody (16B4) is a mouse monoclonal IgM provided at 50 µg/0.5 ml
    • raised against phosphoserine
    • recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP
    • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of p-Ser (16B4): sc-81514.
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
p-Ser Antibody (16B4) sc-81514 50 µg/0.5 ml $279.00

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p-Ser Antibody (16B4) Product Citations

See how others have used p-Ser Antibody (16B4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 28 total

PMID: # 27684411
Krishnan, A. et al. 2017. Oncogenic Actions of SKP2 Involves Deregulation of CDK1 Turnover Mediated by FOXM1. J. Cell. Biochem.. 118: 797-807.

PMID: # 28306139
Wang, X. et al. 2017. The TIR/BB-loop mimetic AS-1 prevents non-alcoholic steatohepatitis and hepatic insulin resistance by inhibiting NLRP3-ASC inflammasome activation. Br. J. Pharmacol.. 174: 1841-1856.

PMID: # 28218425
Shao, Y. et al. 2017. Erlotinib has tumor inhibitory effect in human retinoblastoma cells. Biomed. Pharmacother.. 85: 479-485.

PMID: # 28078537
Wang, Y. et al. 2017. SIRT2-mediated FOXO3a deacetylation drives its nuclear translocation triggering FasL-induced cell apoptosis during renal ischemia reperfusion. Apoptosis.

PMID: # 28658303
Amara, S. et al. 2017. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS ONE. 12: e0180097.

PMID: # 27702664
Baravalle, R. et al. 2017. Impact of R264C and R264H polymorphisms in human aromatase function. J. Steroid Biochem. Mol. Biol.. 167: 23-32.

PMID: # 26102503
Yi Zhen Deng,#1,2,* Ziwei Qu,#1 and Naweed I. Naqvi1,3,* et al. 2015. Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development PLoS Pathog. . 11: e1004972. .

PMID: # 25897829
Fang, XY. et al. 2015. PRL-3 Promotes the Malignant Progression of Melanoma via Triggering Dephosphorylation and Cytoplasmic Localization of NHERF1. J. Invest. Dermatol.. 135: 2273-82.

PMID: # 25389050
Meng, N. et al. 2015. Heterogeneous nuclear ribonucleoprotein E1 regulates protein disulphide isomerase translation in oxidized low-density lipoprotein-activated endothelial cells. Acta physiologica (Oxford, England). 213: 664-75.

PMID: # 25633958
Mangum, LC. et al. 2015. Organochlorine insecticides induce NADPH oxidase-dependent reactive oxygen species in human monocytic cells via phospholipase A2/arachidonic acid. Chem. Res. Toxicol.. 28: 570-84.

Citations 1 to 10 of 28 total

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Asked by: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Answered by: Tech Service
Date published: 2017-06-08

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Asked by: constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Answered by: Technical Support
Date published: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Asked by: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 5 out of 5 by from Great results The results are quit stable. Exposure a little bit longer, you will get very clear ban.
Date published: 2017-10-03
Rated 5 out of 5 by from Used in WB Works well for WB, strong bands is clear and specifi
Date published: 2017-09-07
Rated 4 out of 5 by from Clean background After trying numerous p-Ser antibodies, this one gave the cleanest background. There was good detection at 1:500 diluted in 5% BSA.
Date published: 2017-09-06
Rated 3 out of 5 by from Not working in WB I use the mouse NIH3T3-L1 cells and western blot with whole cell lysates. It does not work.
Date published: 2017-08-22
Rated 5 out of 5 by from good sample We tried it in WB, the nonspecific background in the experiment, happy with it
Date published: 2017-05-18
Rated 1 out of 5 by from no signal in my membrane I tried twice and used 1:300 dilution to detect the P-ser in HEK293T total lyste. but I can see nothing eventhrough I exposed the membrane in Bio-rad Imager more than 15min. it really made me upset
Date published: 2017-05-13
Rated 5 out of 5 by from Good Single strong band in WB(1/200) in canine spleen tissue extract, very good.
Date published: 2017-01-05
Rated 4 out of 5 by from Works well This antibody is well published for use in Western Blot and IP.
Date published: 2016-12-23
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