Optineurin Antibody (C-2) recommended for detection of Optineurin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Optineurin Antibody (C-2): sc-166576

Optineurin Antibody (C-2) is rated 4.7 out of 5 by 6.
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Datasheets
    • Optineurin Antibody (C-2) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against a peptide mapping near the N-terminus of Optineurin of human origin
    • recommended for detection of Optineurin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-166576 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Optineurin (C-2): sc-166576.
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请问c-1产品和c-2产品在WB的应用当中具体有什么不同?

Asked by: 晓晓晓晓晓晓
你好!欢迎咨询圣克鲁斯生物技术(上海)有限公司。如需帮助,请致电021-6093-6350,或可发送邮件至asia@scbio.cn详询,谢谢。
Answered by: Technical Support 8
Date published: 2018-01-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with sc-166576: Optineurin (C-2)?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Optineurin (C-2): sc-166576 monoclonal antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12

Hi Santa Cruz staff, I wish to make a truncated mutant and wondered what more information I could obtain of the peptide sequence used to raise the antibody, for example how close to the N-term is the sequence? Thanks, Colin

Asked by: Colin00000
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Rated 5 out of 5 by from Great features I bought it about a month ago, it band showed good for publication.
Date published: 2017-10-30
Rated 3 out of 5 by from Best anti-Optineurin antibody so far I have been trying to detect human optineurin in whole cell lysates of Jurkat T cells for a while now. I have tried one other Santa Cruz antibody and one from Abcam. This antibody (C-2) seems to give me the best results. I used it at a 1:250 concentration which gave me some background bands, so I think it could go up to 1:500 or 1:1000. One thing I have noticed is that all the antibodies I have used gives me a band around 55-60 kD, which is much smaller than the predicted molecular weight. If anyone else has seen this size discrepancy, please email me.
Date published: 2017-03-13
Rated 5 out of 5 by from Strong western and IHC Very nice product for western blot and IHC staining !!
Date published: 2017-01-12
Rated 5 out of 5 by from Versatile Antibody The Optineurin Antibody (C-2): sc-166576 works well for an array of applications, including Western blot, immunohistochemistry, immunofluorescence, with verification through multiple publications.
Date published: 2016-12-19
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-02-24
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