Ob-R Antibody (B-3)
  • Ob-R Antibody (B-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 870-894 of the short form of Ob-R of mouse origin
  • recommended for detection of short and long forms of Ob-R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
  • available conjugated to agarose (sc-8391 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-8391 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-8391 PE), fluorescein (sc-8391 FITC), Alexa Fluor® 488 (sc-8391 AF488) or Alexa Fluor® 647 (sc-8391 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • blocking peptide, sc-8391 P
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

Ob-R Antibody (B-3): sc-8391

Ob-R Antibody (B-3) is rated 4.9 out of 5 by 11.
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Datasheets
    • Ob-R Antibody (B-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 870-894 of the short form of Ob-R of mouse origin
    • recommended for detection of short and long forms of Ob-R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
    • available conjugated to agarose (sc-8391 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-8391 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-8391 PE), fluorescein (sc-8391 FITC), Alexa Fluor® 488 (sc-8391 AF488) or Alexa Fluor® 647 (sc-8391 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • blocking peptide, sc-8391 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Ob-R Antibody (B-3) sc-8391 200 µg/ml $279.00
Ob-R Antibody (B-3) AC sc-8391 AC 500 µg/ml, 25% ag $366.00
Ob-R Antibody (B-3) Alexa Fluor® 488 sc-8391 AF488 200 µg/ml $314.00
Ob-R Antibody (B-3) Alexa Fluor® 647 sc-8391 AF647 200 µg/ml $314.00
Ob-R Antibody (B-3) FITC sc-8391 FITC 200 µg/ml $292.00
Ob-R Antibody (B-3) HRP sc-8391 HRP 200 µg/ml $279.00
Ob-R (B-3) Blocking Peptide sc-8391 P 100 µg/0.5 ml $61.00
Ob-R Antibody (B-3) PE sc-8391 PE 200 µg/ml $303.00

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Ob-R Antibody (B-3) Product Citations

See how others have used Ob-R Antibody (B-3). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 60 total

PMID: # 27886379
Bougaret, L. et al. 2016. Supernatants of Adipocytes From Obese Versus Normal Weight Women and Breast Cancer Cells: In Vitro Impact on Angiogenesis. J. Cell. Physiol..

PMID: # 27716396
Malaise, O. et al. 2016. Glucocorticoid-induced leucine zipper (GILZ) is involved in glucocorticoid-induced and mineralocorticoid-induced leptin production by osteoarthritis synovial fibroblasts. Arthritis Res Ther.. 18:

PMID: # 26926121
Guo, DF. et al. 2016. The BBSome Controls Energy Homeostasis by Mediating the Transport of the Leptin Receptor to the Plasma Membrane. PLoS genetics. 12: e1005890.

PMID: # 26584065
2016. Insulin and Leptin Signaling in Placenta from Gestational Diabetic Subjects. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme. 48: 62-9.

PMID: # 26147886
Choi, E. et al. 2015. Implication of Leptin-Signaling Proteins and Epstein-Barr Virus in Gastric Carcinomas. PloS one. 10: e0130839.

PMID: # 24899470
Tsai, PJ. et al. 2015. Systemic and placental leptin and its receptors in pregnancies associated with obesity. Reproductive sciences (Thousand Oaks, Calif.). 22: 189-97.

PMID: # 26318657
Charlier, E. et al. 2015. Restriction of spontaneous and prednisolone-induced leptin production to dedifferentiated state in human hip OA chondrocytes: role of Smad1 and β-catenin activation. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society.

PMID: # 24569072
Lee, KN. et al. 2014. The role of leptin in gastric cancer: clinicopathologic features and molecular mechanisms. Biochemical and biophysical research communications. 446: 822-9.

PMID: # 24719379
Li, H. et al. 2014. Perivascular adipose tissue-derived leptin promotes vascular smooth muscle cell phenotypic switching via p38 mitogen-activated protein kinase in metabolic syndrome rats. Experimental biology and medicine (Maywood, N.J.). 239: 954-965.

PMID: # 24958593
Higurashi, T. et al. 2014. Conditional knockout of the leptin receptor in the colonic epithelium revealed the local effects of leptin receptor signaling in the progression of colonic tumors in mice. Carcinogenesis. 35: 2134-41.

Citations 1 to 10 of 60 total

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-01-09
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Rated 5 out of 5 by from High quality Nice wb and ihc;better than polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from Reliable cancer cell staining Detects Ob-R in human cancer cell lines by IHC(P) and western blot very well.
Date published: 2016-12-22
Rated 5 out of 5 by from Good to detect even endogenous low levels of LepR This antibody works quite well to detect the leptin receptor (LepR) of human and mouse origin even in tissues with low level of expression such as the adipose tissue. It give a complex pattern of band due to the complex alternative splicing of the LepR gene
Date published: 2016-12-21
Rated 5 out of 5 by from Antibody was diluted 1:1000 in PBS Antibody was diluted 1:1000 in PBS
Date published: 2015-10-16
Rated 4 out of 5 by from Works well in Western Blot Works well in Western Blot. Does see additional bands, but confirmed specificity using the blocking peptide. Routinely ordered.
Date published: 2015-07-28
Rated 5 out of 5 by from Published for WB and IHC in rabbit ovary Published for WB and IHC in rabbit ovary. -SCBT Publication Review
Date published: 2015-06-19
Rated 5 out of 5 by from Strong band in WB in COLO 320DM whole cell lysate Strong band in WB in COLO 320DM whole cell lysate. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Good publishable data in IHC in human gastric Good publishable data in IHC in human gastric mucosa. -SCBT Publication Review
Date published: 2015-04-23
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