o-Phenanthroline monohydrateA chelating agent for Fe2+

o-Phenanthroline monohydrate (CAS 5144-89-8)

o-Phenanthroline monohydrate | CAS 5144-89-8 is rated 5.0 out of 5 by 1.
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Synonym: 1,10-Phenanthroline
Application: A chelating agent for Fe2+
CAS Number: 5144-89-8
Purity: ≥99%
Molecular Weight: 198.22
Molecular Formula: C12H8N2•H2O
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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o-Phenanthroline is a metal chelating agent that prevents the induction of chromosomal aberrations in streptozotocin-treated cells. Acts as a general MMP (matrix metalloproteinase) inhibitor. Forms a red chelate with ferrous (iron II) ion that absorbs maximally at 510 nm. o-Phenanthroline has been employed as a photometric reagent for iron. o-Phenanthroline forms a complex with Fe2+, but not with Fe3+. NH2OH can be added to the solution to reduce the Fe3+ to Fe2+ for analysis of iron. Due to the o-Phenanthroline's strong UV absorbance, it may interfere with spectrophotometric assays. When complexed with copper, it possesses nuclease activity that has been employed in studying DNA-protein interactions.


References

1. Sigman, D.S., et al. 1991. Meth. Enzymol. 208: 414-433. PMID: 1779842
2. McCarty, R.E. 1992. Anal. Biochem. 205: 371-372. PMID: 1443587
3. Uhm, J.H., et al. 1996. Clin. Exp. Metastasis. 14: 421-433. PMID: 8871536
4. Krane, S.M., et al. 1996. J. Biol. Chem. 271: 28509-28515. PMID: 8910479
5. Wang, Y., et al. 1999. J. Biol. Chem. 274: 33043-33049. PMID: 10551873
6. Harayama, T., et al. 1999. Jpn. J. Cancer Res. 90: 942-950. PMID: 10551322
7. Bolzán, A.D., et al. 2000. Mutat. Res. 447: 221-226. PMID: 10751605
8. Stix, B., et al. 2001. Am. J. Pathol. 159: 561-570. PMID: 11485914

Physical State :
Solid
Solubility :
Soluble in water (3 mg/ml), ethanol (25 mg/ml), DMSO (100 mg/ml), acetone, and benzene (70 parts).
Storage :
Store at room temperature
Melting Point :
100-104° C (lit.)
Refractive Index :
n20D 1.26
pK Values :
pKb: 5.16
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SF8437000
PubChem CID :
21226
Merck Index :
14: 7214
MDL Number :
MFCD00149973
EC Number :
200-629-2
Beilstein Registry :
40080961
SMILES :
C1=CC2=C(C3=C(C=CC=N3)C=C2)N=C1.O

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from o o-Phenanthroline monohydrate was reported in many articles as a nuclease to study DNA interactions. -SCBT Publication Review
Date published: 2015-04-13
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