Nonidet® P40 (substitute) is a non-ionic, non-denaturing detergent suitable for solubilizing and isolation of membrane proteins. Has been used to solubilize cerebral GABA receptors. Also useful in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors. Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
1. Stephens, R.E. 1985. J. Cell Biol. 100: 1082-1090. PMID: 3980579 2. Schraag, B., et al. 1993. Anal. Biochem. 211: 233-239. PMID: 7686354 3. D'Arrigo, A., et al. 1993. J. Biol. Chem. 268: 2802-2808. PMID: 8428954
Soluble in water.
Store at room temperature
250° C (lit.)
1.06 g/cm3 at 20
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
UN 3082, Class 9, Packing group III
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Nonidet P40 (stitute)
See how others have used Nonidet P40 (stitute). Click on the entry to view the PubMed entry
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Rated 5 out of
Kellner et alKellner et al. (PubMed ID 25148723) used Nonidet P40 to stain human monocytes in order to visualize lipopolyshaccharide-stimulated toxicity and inflammatory response. -SCBT Publication Review
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