Necrostatin-1 An inhibitor of the necroptosis cell death pathway

Necrostatin-1 (CAS 4311-88-0)

Necrostatin-1 | CAS 4311-88-0 is rated 4.7 out of 5 by 3.
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| See product citations (32)
Synonym: Methylthiohydantoin-DL-tryptophan
Application: An inhibitor of the necroptosis cell death pathway
CAS Number: 4311-88-0
Purity: ≥95%
Molecular Weight: 259.32
Molecular Formula: C13H13N3OS
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Necrostatin-1 sc-200142 20 mg $80.00
Necrostatin-1 sc-200142A 100 mg $320.00

Necrostatin-1 is a potent and selective inhibitor of necroptosis, a mechanism of programmed cell death separate from apoptosis. Necrostatin-1 functions as an allosteric inhibitor of the death domain receptor-associated adaptor kinase RIP (RIP1) in the necroptosis pathway. While affecting this distinct cell death pathway, Necrostatin-1 does not present any perturbation of the Fas/TNFR triggered canonical apoptosis cascade.


References

1. Degterev, A., et al. 2005. Nat. Chem. Biol. 1: 112-119. PMID: 16408008
2. Degterev, A., et al. 2008. Nat. Chem. Biol. 4: 313-321. PMID: 18408713
3. He, W. et al. 2014. Oncogene. 33(23): 3004-3013. PMID: 23831571

Physical State :
Solid
Solubility :
Soluble in DMSO (10-25 mg/ml), 100% ethanol (6 mg/ml), and methanol (5 mg/ml).
Storage :
Store at 4° C
Boiling Point :
~441.9° C at 760 mmHg (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.74
IC50 :
the loss of mitochondrial membrane potential: EC5050 = 490 nM (TNFα-treated Jurkat cells); endogenous RIP1 autophosphorylation : EC5050 = 182 nM (human Jurkat cells)
pK Values :
pKa: 9.46, pKb: 0.63
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
2828334
MDL Number :
MFCD00056916
SMILES :
CN1C(=O)C(NC1=S)CC2=CNC3=CC=CC=C32

Download SDS (MSDS)

Certificate of Analysis

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Necrostatin-1Product Citations

See how others have used Necrostatin-1. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 32 total

PMID: # 28968601
Fan, B. et al. 2017. Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer. Cell. Physiol. Biochem.. 43: 1003-1011.

PMID: # 26911899
Tian, F. et al. 2016. 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Inhibits RIPK1/RIPK3-Dependent Necroptosis in THP-1-Derived Foam Cells. Scientific reports. 6: 21992.

PMID: # 26584462
Zeng, F. et al. 2016. Use of the rainbow trout cell lines, RTgill-W1 and RTL-W1 to evaluate the toxic potential of benzotriazoles. Ecotoxicology and environmental safety. 124: 315-23.

PMID: # 26787898
Guha, P. et al. 2016. Cocaine elicits autophagic cytotoxicity via a nitric oxide-GAPDH signaling cascade. Proceedings of the National Academy of Sciences of the United States of America. 113: 1417-22.

PMID: # 26935990
Wang, W. et al. 2016. Activation of innate antiviral immune response via double-stranded RNA-dependent RLR receptor-mediated necroptosis. Scientific reports. 6: 22550.

PMID: # 26936128
Tian, W. et al. 2016. mTOR signaling pathway is inhibited downstream of the cyclophilin D-mediated mitochondrial permeability transition in honokiol-triggered regulated necrosis. Mol Med Rep. 13: 3227-35.

PMID: # 26259235
Wang, Y. et al. 2015. PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death. Oncotarget. 6: 25252-65.

PMID: # 26100518
Lin, PH. et al. 2015. N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting. J. Biomed. Sci.. 22: 44.

PMID: # 26000607
Zhang, M. et al. 2015. The Roles of ROS and Caspases in TRAIL-Induced Apoptosis and Necroptosis in Human Pancreatic Cancer Cells. PloS one. 10: e0127386.

PMID: # 26097885
Eling, N. et al. 2015. Identification of artesunate as a specific activator of ferroptosis in pancreatic cancer cells. Oncoscience. 2: 517-32.

Citations 1 to 10 of 32 total

Can this product be stored in solution?

Asked by: hawkeye11
This chemical, Necrostatin-1 (CAS 4311-88-0), can be stored in DMSO solution at -20ºC. It is stable up to 3 months stored at -20ºC.
Answered by: Chemical Support 1
Date published: 2018-01-15

What is the solubility of this product?

Asked by: hawkeye11
This chemical, Necrostatin-1 (CAS 4311-88-0), is soluble in DMSO: ~10mg/ml, Methanol: ~5mg/ml and 100% Ethanol: ~6mg/ml.
Answered by: Chemical Support 1
Date published: 2017-03-06
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Rated 4 out of 5 by from This inhibitor works well We used this inhibitor to pretreat THP1 cells for 1 h and stimulated with pore-forming toxins, it blocked the LDH release.
Date published: 2018-05-24
Rated 5 out of 5 by from Produced optimal results in our cell culture Produced optimal results in our cell culture.
Date published: 2015-09-23
Rated 5 out of 5 by from He He, et. al. (PubMed ID 23831571) used Necrostatin-1 (SCBT Catalog # sc-200142) to block the necroptosis pathway in A549 and UM-UC-3 cells, thus adequately blocking the cytotoxicity effect of Chal-24. -SCBT Publication Review
Date published: 2015-03-15
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