Necrostatin-1 is a potent and selective inhibitor of necroptosis, a mechanism of programmed cell death separate from apoptosis. Necrostatin-1 functions as an allosteric inhibitor of the death domain receptor-associated adaptor kinase RIP (RIP1) in the necroptosis pathway. While affecting this distinct cell death pathway, Necrostatin-1 does not present any perturbation of the Fas/TNFR triggered canonical apoptosis cascade.
1. Degterev, A., et al. 2005. Nat. Chem. Biol. 1: 112-119. PMID: 16408008 2. Degterev, A., et al. 2008. Nat. Chem. Biol. 4: 313-321. PMID: 18408713 3. He, W. et al. 2014. Oncogene. 33(23): 3004-3013. PMID: 23831571
Soluble in DMSO (10-25 mg/ml), 100% ethanol (6 mg/ml), and methanol (5 mg/ml).
Store at 4° C
~441.9° C at 760 mmHg (Predicted)
~1.4 g/cm3 (Predicted)
the loss of mitochondrial membrane potential: EC5050 = 490 nM (TNFα-treated Jurkat cells); endogenous RIP1 autophosphorylation : EC5050 = 182 nM (human Jurkat cells)
pKa: 9.46, pKb: 0.63
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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See how others have used Necrostatin-1. Click on the entry to view the PubMed entry
Rated 4 out of
This inhibitor works wellWe used this inhibitor to pretreat THP1 cells for 1 h and stimulated with pore-forming toxins, it blocked the LDH release.
Date published: 2018-05-24
Rated 5 out of
Produced optimal results in our cell cultureProduced optimal results in our cell culture.
Date published: 2015-09-23
Rated 5 out of
HeHe, et. al. (PubMed ID 23831571) used Necrostatin-1 (SCBT Catalog # sc-200142) to block the necroptosis pathway in A549 and UM-UC-3 cells, thus adequately blocking the cytotoxicity effect of Chal-24. -SCBT Publication Review
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