Necrostatin-1 An inhibitor of the necroptosis cell death pathway

Necrostatin-1 (CAS 4311-88-0)

Necrostatin-1 | CAS 4311-88-0 is rated 4.7 out of 5 by 3.
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Synonym: Methylthiohydantoin-DL-tryptophan
Application: An inhibitor of the necroptosis cell death pathway
CAS Number: 4311-88-0
Purity: ≥95%
Molecular Weight: 259.32
Molecular Formula: C13H13N3OS
* Refer to Certificate of Analysis for lot specific data (including water content).
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Necrostatin-1 is a potent and selective inhibitor of necroptosis, a mechanism of programmed cell death separate from apoptosis. Necrostatin-1 functions as an allosteric inhibitor of the death domain receptor-associated adaptor kinase RIP (RIP1) in the necroptosis pathway. While affecting this distinct cell death pathway, Necrostatin-1 does not present any perturbation of the Fas/TNFR triggered canonical apoptosis cascade.


References

1. Degterev, A., et al. 2005. Nat. Chem. Biol. 1: 112-119. PMID: 16408008
2. Degterev, A., et al. 2008. Nat. Chem. Biol. 4: 313-321. PMID: 18408713
3. He, W. et al. 2014. Oncogene. 33(23): 3004-3013. PMID: 23831571

Physical State :
Solid
Solubility :
Soluble in DMSO (10-25 mg/ml), 100% ethanol (6 mg/ml), and methanol (5 mg/ml).
Storage :
Store at 4° C
Boiling Point :
~441.9° C at 760 mmHg (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.74
IC50 :
the loss of mitochondrial membrane potential: EC5050 = 490 nM (TNFα-treated Jurkat cells); endogenous RIP1 autophosphorylation : EC5050 = 182 nM (human Jurkat cells)
pK Values :
pKa: 9.46, pKb: 0.63
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
2828334
MDL Number :
MFCD00056916
SMILES :
CN1C(=O)C(NC1=S)CC2=CNC3=CC=CC=C32

Download SDS (MSDS)

Certificate of Analysis

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Necrostatin-1  Product Citations

See how others have used Necrostatin-1. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 32 total

PMID: # 28968601  Fan, B. et al. 2017. Cell. Physiol. Biochem. 43: 1003-1011.

PMID: # 26911899  Tian, F. et al. 2016. Scientific reports. 6: 21992.

PMID: # 26584462  Zeng, F. et al. 2016. Ecotoxicology and environmental safety. 124: 315-23.

PMID: # 26787898  Guha, P. et al. 2016. Proceedings of the National Academy of Sciences of the United States of America. 113: 1417-22.

PMID: # 26935990  Wang, W. et al. 2016. Scientific reports. 6: 22550.

PMID: # 26936128  Tian, W. et al. 2016. Mol Med Rep. 13: 3227-35.

PMID: # 26259235  Wang, Y. et al. 2015. Oncotarget. 6: 25252-65.

PMID: # 26100518  Lin, PH. et al. 2015. J. Biomed. Sci. 22: 44.

PMID: # 26000607  Zhang, M. et al. 2015. PloS one. 10: e0127386.

PMID: # 26097885  Eling, N. et al. 2015. Oncoscience. 2: 517-32.

Citations 1 to 10 of 32 total

Can this product be stored in solution?

Asked by: hawkeye11
This chemical, Necrostatin-1 (CAS 4311-88-0), can be stored in DMSO solution at -20ºC. It is stable up to 3 months stored at -20ºC.
Answered by: Chemical Support 1
Date published: 2018-01-15

What is the solubility of this product?

Asked by: hawkeye11
This chemical, Necrostatin-1 (CAS 4311-88-0), is soluble in DMSO: ~10mg/ml, Methanol: ~5mg/ml and 100% Ethanol: ~6mg/ml.
Answered by: Chemical Support 1
Date published: 2017-03-06
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Rated 4 out of 5 by from This inhibitor works well We used this inhibitor to pretreat THP1 cells for 1 h and stimulated with pore-forming toxins, it blocked the LDH release.
Date published: 2018-05-24
Rated 5 out of 5 by from Produced optimal results in our cell culture Produced optimal results in our cell culture.
Date published: 2015-09-23
Rated 5 out of 5 by from He He, et. al. (PubMed ID 23831571) used Necrostatin-1 (SCBT Catalog # sc-200142) to block the necroptosis pathway in A549 and UM-UC-3 cells, thus adequately blocking the cytotoxicity effect of Chal-24. -SCBT Publication Review
Date published: 2015-03-15
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