N,Nprime-Methylenebis-Acrylamide, 2% A preparatory agent for polyacrylamide gels

N,N′-Methylenebis-Acrylamide, 2% (CAS 110-26-9)

N,N′-Methylenebis-Acrylamide, 2%, CAS 110-26-9 is rated 5.0 out of 5 by 1.
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Application: A preparatory agent for polyacrylamide gels
CAS Number: 110-26-9
Molecular Weight: 154.17
Molecular Formula: C7H10N2O2
* Refer to Certificate of Analysis for lot specific data (including water content).
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N,N′-Methylenebis-Acrylamide, 2% (bis-Acrylamide) is used in the preparation of polyacrylamide gels for electrophoretic separation of nucleic acids and proteins. Recommended for use with Acrylamide Solution, 40% (sc-3721). Acrylamide forms linear polymers and bis-Acrylamide introduces cross-links between polyacrylamide chains; by mixing the appropriate ratio of Acrylamide to bis-Acrylamide, the optimal pore size can be obtained for the protein being resolved. Ready-to-use acrylamide solutions offer convenience and eliminate the risks associated with the handling of acrylamide powder. Polymerization agents, TEMED (sc-29111) and APS (Ammonium persulfate, sc-202946) are also available. For denaturing gels, SDS (20% Solution) (sc-24950) is available.


References

1. Raymond, S. and Aurell, B. 1962. Science. 138: 152-153. PMID: 17818402
2. Vesterberg, O. 1993. Electrophoresis. 14: 1243-1249. PMID: 8137783
3. Beeley, J.A., et al. 1996. Electrophoresis. 17: 505-506. PMID: 8740168
4. Langen, H., et al. 1997. Electrophoresis. 18: 2085-2090. PMID: 9420174
5. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924
6. Rabilloud, T. 2002. Proteomics. 2: 3-10. PMID: 11788986

Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
RTECS :
AS3678000
PubChem CID :
8041
MDL Number :
MFCD00008625
SMILES :
C=CC(=O)NCNC(=O)C=C

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Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite the use of N Various publications cite the use of N,N -Methylenebis-Acrylamide, 2% (bis-Acrylamide) in the preparation of polyacrylamide gels for electrophoretic separation of nucleic acids and proteins. Recommended for use with Acrylamide Solution, 40% (sc-3721). -SCBT Publication Review
Date published: 2015-03-22
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