Myriocin is a small-molecule inhibitor of serine palmitoyltransferase (SPTLC), isolated from the Mycelia sterilia thermophilic fungus, with immunosuppressive properties. SPTLC is responsible for catalyzing the committed step of sphingolipid biosynthesis; inhibition of this activity by myriocin leads to a depletion of sphingolipid levels in the cell. Apoptosis of interleukin-2-dependent cytotoxic T cells (CTLL-2) is induced by exposure to myriocin, and because this observed cell death is suspendable with addition of sphingosine, it is suggested that cellular levels of sphingolipids are important for signaling the onset of and escape from apoptosis in CTLL-2 T cells. This same observation indicates a mechanism for the immunosuppression associated with myriocin. Inhibition of sphingolipid production by myriocin is thought to disturb the composition of the lipid raft structure crucial for hepatitis C virus replication, indirectly suppressing proliferation of the virus. Adhesion to the extracellular matrix is shown to be disrupted in cells depleted of sphingolipids, and myriocin represents a tool for studying such behaviors of cells in sphingolipid-depleted states.
1. Fujita, T., et al. 1994. J. Antibiot. 47: 208-215. PMID: 8150717 2. Miyake, Y., et al. 1995. Biochem. Biophys. Res. Commun. 211: 396-403. PMID: 7794249 3. Hidari KIPJ, S., et al. 1996. J. Biol. Chem. 271: 14636-14641. PMID: 8662871 4. Nakamura, S., et al. 1996. J. Biol. Chem. 271: 1255-1257. PMID: 8576106 5. Umehara, T., et al. 2006. Biochem. Biophys. Res. Commun. 346: 67-73. PMID: 16750511
Soluble in DMSO (heat briefly in boiling water bath) (25 mg/ml), dilute base (50 mM NaOH) (~5 mg/ml), and methanol (2 mg/ml). Insoluble in water.
Thank you for your question. We do not test in-house the activity of this compound, Myriocin (ISP-1) (CAS 35891-70-4). However, below are some references that may contain relevant information to help address your question.
Hanada, K., et al. 2000. Biochem. Pharmacol. 59, 1211.
Chen, J.K., et al. 1999. Chem. Biol. 6, 221.
Hidari, K.I.P.J., et al. 1996. J. Biol. Chem. 271, 14636.
Nakamura, S., et al. 1996. J. Biol. Chem. 271, 1255.
Mikaye, Y., et al. 1995. Biochem. Biophys. Res. Commun. 211, 396.
Fujita, T., et al. 1994. J. Antibiot. 47, 208.
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S ghyS ghy, . et al. (PubMed 26238178) disrupted lipid rafts by cleaving sphingomyelin with sphingomyelinase, cholesterol depletion with methyl -cyclodextrin and ganglioside breakdown with Myriocin (ISP-1), an inhibitor of sphingolipid biosynthesis with immunosuppressive properties. -SCBT Publication Review
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