mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 790 (CFL 790)
supplied at 200 µg in 0.5 ml volume
CruzFluor™ 790 (CFL 790) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 790, offering comparable photostability and the ability to resist protein quenching
Excitation of 782 nm, Emission of 811 nm
Suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Western blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM)
Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 790 (sc-516181)
For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 790 (sc-516195) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 790 (CFL 790) reagents, please contact Technical Service.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mouse IgGλ light chain binding protein (m-IgGλ BP) conjugated to horseradish peroxidase (HRP) is suitable for binding to mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain primary antibodies. Mouse IgGλ light chain binding protein is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB), immunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGλ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. CruzFluor™ 790 is an infrared fluorescent dye that is an excellent substitute for AlexFluor® 790.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
Hi, Am still waiting to process my data before I write my review. Just to clarify this my idea, I used both the lambda and kappa of the m-IgG BP-CFL 790 together as secondary to detect my primary antibodies. Is that a problem in any way? My result was +
Asked by: Chrisiyke
Thank you for your question. For two-color Western blots using primary antibodies with kappa or lambda light chains, primary antibodies and their corresponding secondary detection reagents may be incubated simultaneously. However, we would not recommend using kappa and lambda CFL 790 conjugates together, since these are the same color. Instead, we would recommend using our CFL 680 (green) and CFL 790 (red) together for two-color detection.
Rated 5 out of
Really great productI really liked being able to do a two-color western with the two monos. I used one kappa mono and one lambda mono. Clean, clear signal with no cross-reactivity. Really was a straight forward experiment!
Date published: 2016-12-05
Rated 5 out of
Worked great for a two-color westernI really liked being able to do a two-color western with two monos, one kappa and one lambda. Clean, clear signal with no cross-reactivity. Makes my experiments very easy!
Date published: 2016-11-30
Rated 5 out of
Very SpecificI was very pleased to see such clean results on my IR Westerns. Excited that multiple fluorochromes are available.
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