m-IgG lambda BP-CFL 680mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Cruz Fluor™ 680, for NIR WB, IF, IHC(P) and FCM

m-IgGλ BP-CFL 680: sc-516194

m-IgGλ BP-CFL 680 is rated 5.0 out of 5 by 4.
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Datasheets
    • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 680 (CFL 680)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 683 nm, Emission of 700 nm
    • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 680 (sc-516180)
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
    • If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 680 (sc-516194) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 680 (CFL 680) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 5 out of 5 by from Good results with this product I regularly use some of the monos that are lambda light chain and this binding protein worked nicely. Very clean signal and no background with my LiCor.
Date published: 2016-12-05
Rated 5 out of 5 by from Good secondary This secondary binding protein gave great IF Western Blot data
Date published: 2016-12-01
Rated 5 out of 5 by from No background! I regularly use a couple of monos that are lambda light chain and this worked nicely. Clean signal and no background with my LiCor.
Date published: 2016-11-30
Rated 5 out of 5 by from No background First time I've used this binding protein for IF WB. The bands were sharp and no background was detected compared to my usual secondary antibody. I will definitely keep using this product.
Date published: 2016-11-30
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