mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 488 (CFL 488)
supplied at 200 µg in 0.5 ml volume
CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
Excitation of 488 nm, Emission of 514 nm
Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 488 (sc-516176)
If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 488 (sc-516190), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 488 (CFL 488) reagents, please contact Technical Service.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mouse IgGλ light chain binding protein (m-IgGλ BP) conjugated to CruzFluor™ 488 (CFL 488) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGλ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGλ light chain binding protein (m-IgGλ BP) is suitable for binding to mouse IgGλ light chain immunoglobulins; not suitable for use with mouse monoclonal IgGκ light chain primary antibodies. CruzFluor™ 488 is a green fluorescent dye that is an excellent substitute for AlexFluor® 488, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
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Rated 1 out of
Amr G from
Did not work for immunofluorescenceDid not work for immunofluorescence. Did not work on mouse cells or human cells.
Date published: 2019-05-01
Rated 4 out of
Tomas Grousl from
no objections so farI've recently tried m-IgG BP-CFL 488 antibody and I am satisfied with the result.
Date published: 2017-11-27
Rated 3 out of
GoodQuite good antibody. The sc-516177 antibody gives better result in IF analysis. Dilution 1:100
Date published: 2017-11-17
Rated 4 out of
Ok resultI tried both kappa and lambda CFL 488 binding proteins as I had both types of antibodies. Loved the specificity of the kappa binding protein. This lambda product gave decent results, but not as sharp as the kappa product.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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