m-IgG lambda BP-CFL 488 mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Cruz Fluor™ 488, for WB (RGB), IF, IHC(P) and FCM

m-IgGλ BP-CFL 488: sc-516190

m-IgGλ BP-CFL 488 is rated 3.0 out of 5 by 4.
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Datasheets
    • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to CruzFluor™ 488 (CFL 488)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 488 (CFL 488) is a green fluorescent dye that is an excellent substitute for Alexa Fluor® 488, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 488 nm, Emission of 514 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-CFL 488 (sc-516176)
    • If you have an interest in testing a 10 µg sample of m-IgGλ BP-CFL 488 (sc-516190), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 488 (CFL 488) reagents, please contact Technical Service.
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Rated 1 out of 5 by from Did not work for immunofluorescence Did not work for immunofluorescence. Did not work on mouse cells or human cells.
Date published: 2019-05-01
Rated 4 out of 5 by from no objections so far I've recently tried m-IgG BP-CFL 488 antibody and I am satisfied with the result.
Date published: 2017-11-27
Rated 3 out of 5 by from Good Quite good antibody. The sc-516177 antibody gives better result in IF analysis. Dilution 1:100
Date published: 2017-11-17
Rated 4 out of 5 by from Ok result I tried both kappa and lambda CFL 488 binding proteins as I had both types of antibodies. Loved the specificity of the kappa binding protein. This lambda product gave decent results, but not as sharp as the kappa product.
Date published: 2016-11-30
  • y_2019, m_5, d_24, h_18
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