m-IgG kappa BP-CFL 790 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 790, for NIR WB, IF, IHC(P) and FCM

m-IgGκ BP-CFL 790: sc-516181

m-IgGκ BP-CFL 790 is rated 5.0 out of 5 by 17.
  • y_2019, m_1, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_516181, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 45ms
  • REVIEWS, PRODUCT
Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 790 (CFL 790)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 790 (CFL 790) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 790, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 782 nm, Emission of 811 nm
    • Suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Western blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-CFL 790 (sc-516181) or m-IgGκ BP-CFL 680 (sc-516180), please contact Technical Service.
Submit a review for this product and receive 15 CruzCredits

Does this product (mouse IgG binding protein) recognize denatured IgG?

Asked by: ChrisB
Thank you for your question. Yes, our mouse IgG kappa binding protein (m-IgGκ BP-CFL 790: sc-516181) does recognize the kappa light chain of denatured IgG.
Answered by: Technical Support
Date published: 2017-02-21
  • y_2019, m_1, d_21, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_516181, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 44ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from m-IgGk BP-CFL790 Worked really well for western blotting producing very clean bands in extracts run from primary human monocytes.
Date published: 2018-04-17
Rated 5 out of 5 by from Brilliant signal I got a sample of CFL790 to try with near IR western blot to image with Licor Odyssey Fc. We saw brilliant signal without much optimization. We used it to detect GFP with mouse anti GFP from SCBT along with CFL790 at 1:1000. Earlier we had to struggle to see signal with Licor IR Dy680. I highly recommend this to save a ton of your time in optimization.
Date published: 2017-11-20
Rated 5 out of 5 by from Clean signal, little or no background This work well for our detection of GAPDH in HEK cells cyoplasmic lysate at 1:2000
Date published: 2017-10-13
Rated 5 out of 5 by from Good Band This antibody's quality is so nice. I got the nice band.
Date published: 2017-08-31
Rated 5 out of 5 by from Very sensitive secondary antibody for WB The secondary antibody was used at 1/3000 to 1/4000 dilutions in 5% nonfat milk, and the signals were detect by an Odyssey scanner. Bands are very clear, and the sensitivity seems better than HRP.
Date published: 2017-05-31
Rated 5 out of 5 by from Good image It is very sensitive and give me a clear band in Western blot.
Date published: 2017-05-15
Rated 5 out of 5 by from Good secondary antibody It worked well with 1:500dilution in the immunohistochemistry.
Date published: 2017-03-18
Rated 5 out of 5 by from Clean signals Tried this fluorescent secondary antibody for Western blot, detecting on LI-COR Odyssey. Gave clean signals with minimal background on nitrocellulose membrane using 5% milk in TBS-T as blocking reagent. Diluted 1:2000, could have used much less.
Date published: 2017-03-15
  • y_2019, m_1, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_516181, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 34ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.