m-IgG kappa BP-CFL 680 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 680, for NIR WB, IF, IHC(P) and FCM

m-IgGκ BP-CFL 680: sc-516180

m-IgGκ BP-CFL 680 is rated 4.8 out of 5 by 17.
  • y_2019, m_7, d_18, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_516180, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 33ms
  • REVIEWS, PRODUCT
Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 680 (CFL 680)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 680 (CFL 680) is a near infrared fluorescent dye that is an excellent substitute for Alexa Fluor® 680, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 683 nm, Emission of 700 nm
    • Suitable for use with Near-Infrared (NIR) imaging systems, such as LI-COR/Odyssey, Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Near-Infrared Western blotting (WB [NIR]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For optimal results, use low fluorescence PVDF membranes, such as Immobilon®-FL PVDF, and UltraCruz® Blocking Reagent (sc-516214) for all blocking, primary antibody and mouse IgG binding protein incubations.
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-CFL 680 (sc-516180) or m-IgGκ BP-CFL 790 (sc-516181), please contact our Technical Service Department (or your local Distributor) for more information.
Submit a review for this product and receive 15 CruzCredits

Does this secondary react with denatured mouse IgG light or heavy chains? Would like to use it in a Co-IP experiment. Thank you.

Asked by: zz1234
This mouse IgG binding protein (secondary) specifically binds to the kappa light chain of mouse IgG, whether it is denatured or not. It will not recognize mouse heavy chain IgG.
Answered by: Technical Support
Date published: 2019-01-06

Is this reagent mouse specific, ie how much cross reactivity would I expect with rat and mouse as primary antibodies.

Asked by: curosity
Thank you for your question. We do not expect this mouse IgG binding protein to have any cross-reactivity with primary antibodies raised in rat, and will cover this by warranty.
Answered by: Technical Service
Date published: 2017-02-09
  • y_2019, m_7, d_18, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_516180, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 34ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Works well Working good in both BSA and casein buffer system (1:1000, 200ug/0.5ml)
Date published: 2018-01-03
Rated 5 out of 5 by from Work good We bought a Santa beta-actin and a srebf1 antibody, they both worked well, so did this anti-antibody sc-516180.
Date published: 2017-09-30
Rated 5 out of 5 by from Works very well This antibody is working very well and I got it that I want.
Date published: 2017-08-23
Rated 4 out of 5 by from Works great Use a lower dilution to avoid too much background.
Date published: 2017-08-03
Rated 5 out of 5 by from Excellent We tested this antibody against LiCOR Donkey anti-mouse-IRdye 680. I think it worked better. There wa a much stronger single... will have to dilute.
Date published: 2017-07-28
Rated 5 out of 5 by from Good Nice specificity. Suitable for Licor. Works at low concentration also.
Date published: 2017-06-08
Rated 5 out of 5 by from strong staining This antibody used for IF staining as a secondary antibody, I got strong signal,which is expected staining.
Date published: 2017-05-24
Rated 5 out of 5 by from Very good! This secondary antibody worked very well in western blot analysis by using Odyssey scanner. Used diluted 1:10000 for 1h at room temperature, the signal was very strong and specific with 50µg protein per well loaded.
Date published: 2017-05-16
  • y_2019, m_7, d_18, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_516180, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 47ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.