mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 647 (CFL 647)
CruzFluor™ 647 (CFL 647) conjugated secondaries are similar to Alexa Fluor® 647 and Cy5® fluorescent dyes, offering comparable photostability and the ability to resist protein quenching
Excitation of 654 nm, Emission of 669 nm
supplied at 200 µg in 0.5 ml volume
Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
If you have an interest in testing a 10 µg sample of m-IgGκ BP-CFL 647 (sc-516179) our highly recommended recombinant alternative to conventional secondary anti-mouse IgG CruzFluor™ 647 (CFL 647) reagents, please contact our Technical Service Department (or your local Distributor) for more information.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Mouse IgGκ light chain binding protein (m-IgGκ BP) conjugated to CruzFluor™ 647 (CFL 647) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for Western Blotting (WB), Immunofluorescence (IF) and Flow Cytometry (FCM) signal enhancement. Mouse IgGκ light chain binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgGκ light chain binding protein (m-IgGκ BP) is suitable for binding to mouse IgGκ light chain immunoglobulins, comprising a vast majority of mouse monoclonal primary antibodies; not suitable for use with mouse monoclonal IgGλ light chain primary antibodies. CruzFluor™ 647 is a far-red fluorescent dye that is an excellent substitute for AlexFluor® 647 or Cy5®, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
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Rated 5 out of
Great for intracellular staining-flow cytometryStained HEK 293T (human) and B16F10 (mouse) cells using this antibody and ran flow cytometry. The staining was clear without much background and the human and mouse cells gave a good Stain Index. The target was Ku70 protein. I used the antibody at a 1:200 dilution.
Date published: 2018-06-18
Rated 5 out of
GreatHighly specific and bright in HeLa cells for IF staining!
Date published: 2017-11-27
Rated 5 out of
Great experience!I called them to get sample for 2-3 antibodies and they shipped it for me right away. It was a great experience\!
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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