m-IgG kappa BP-CFL 594 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 594, for WB (RGB), IF, IHC(P) and FCM

m-IgGκ BP-CFL 594: sc-516178

m-IgGκ BP-CFL 594 is rated 5.0 out of 5 by 11.
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Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 594 (CFL 594)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 594 (CFL 594) is a red fluorescent dye that is an excellent substitute for Alexa Fluor® 594, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 587 nm, Emission of 603 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
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Is there data available supporting the claims that CruzFluor dyes have better photostability than their Alexa Fluor counterparts?

Asked by: science_man
Our CruzFluor™ dyes offer comparable photostability and the ability to resist protein quenching to their Alexa Fluor® counterparts.
Answered by: Technical Support
Date published: 2019-02-10

Can m-IgGκ BP-CFL 594 (sc-516178) antibody be used for fluorescent western blot? Thanks.

Asked by: Elexa
Thank you for your question. We have not yet tested any of our CruzFluor™ 594 (CFL 594) conjugates for Western blot detection. If you have the correct instrument for detection of this fluorescent wavelength in a Western blot, then yes our mouse IgG kappa binding protein (m-IgGκ BP) would be suitable. We are currently offering a free 10 µg sample of m-IgGκ BP-CFL 594: sc-516178. Please contact our Technical Service Department if you would like to receive a sample. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-07-11

Hello, What is the molecular weight of product# sc-516178 (m-IgGκ BP-CFL 594)? Thanks.

Asked by: Randall Lowe
Thank you for your question. The molecular weight of the mouse IgG binding protein is ~240 kDa.Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you would like to try a sample of it or if you have any further questions!
Answered by: Tech Service
Date published: 2017-06-28

When should a mouse IgG Binding Protein be used instead of a conventional secondary antibody and what are the advantages of the binding protein?

Asked by: lpk346
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins in any application when you are using a mouse monoclonal primary antibody to detect your target antigen. The Mouse IgG Binding Proteins are designed to recognize a specific epitope on either the kappa or lambda light chain of mouse IgG. As a result, they are a highly specific reagent that provides strong signal with minimal background or non-specific binding, compared with a conventional secondary antibody. In addition, because they are not raised in a host animal, there is no lot-to-lot variation which is associated with conventionally generated secondary antibodies.
Answered by: Technical Support
Date published: 2016-12-14
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Rated 5 out of 5 by from Works great as a secondary alternative I bought this product for detection of mouse IgG and confirm IgG leakage following brain viral injection. Also works great as a secondary antibody to detect mouse primary antibodies for IF staining in both cell prep and tissue.
Date published: 2018-03-14
Rated 5 out of 5 by from Good for cryosections I received a sample of the m-IgGκ BP-CFL 594 (sc-516178). I have tested it with both chromogranin A (sc-393941) and PNMT (sc-393995) and they work fine for immunofluorescence of cryosections of mouse adrenal at 1/60 dilution of primary and 1/80 dilution of secondary. I had similar results for the FITC aquivalent (sc-516140).
Date published: 2017-11-24
Rated 5 out of 5 by from Good IF signal Used at 1:200 on methanol fixed cells stained with a primary antibody. Incubated for 1 h at RT.
Date published: 2017-09-15
Rated 5 out of 5 by from great antibody for immunohistochemistry, 1:500 worked well using tissue extracted from the brain.
Date published: 2017-07-04
Rated 5 out of 5 by from This is a good second antibody! I bought it before three days, Immunofluorescence (IF) is very good! I use NSCs( neural stem cells) , primary antibody is GFAP, dilution 1:100.
Date published: 2017-02-28
Rated 5 out of 5 by from Great for IF staining! Our data using this CFL 594 binding protein is really nice and clean. Using our standard protocol, we get really specific straining without non-specific background.
Date published: 2016-12-14
Rated 5 out of 5 by from Highly recommended alternative to AF594 secondary This is a highly recommended alternative to Alexa Fluor 594 conjugated secondary antibodies. It offers a bright red stain for IF or FCM, and is highly specific for binding the kappa light chains of mouse IgG.
Date published: 2016-12-14
Rated 5 out of 5 by from Exciting new product Compared to our conventional CFL 594 conjugated secondary antibodies, our QC results for this new binding protein in IF staining was very specific with little background. Using this binding protein eliminates the lot to lot variation seen with our polyclonal secondary antibodies, yielding more consistent data.
Date published: 2016-12-13
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