MES A zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays

MES (CAS 4432-31-9)

MES | CAS 4432-31-9 is rated 5.0 out of 5 by 1.
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Synonym: 2-(N-Morpholino)ethanesulfonic acid hydrate; 4-Morpholineethanesulfonic acid monohydrate
Application: A zwitterionic buffer effective in the pH range of 6-8, used in various protein characterization assays
CAS Number: 4432-31-9
Purity: ≥99%
Molecular Weight: 195.24
Molecular Formula: C6H13NO4S
* Refer to Certificate of Analysis for lot specific data (including water content).
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MES is a zwitterionic N-substituted aminosulfonic acid known as a Good's buffer, active in the pH range 6-8 with a pKa of 6.10 at 25°C. With a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. MES is used as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide.

Also available: MES, Sodium Salt (sc-216095); MES hemisodium salt (sc-216093); MES solution (sc-300959); MES monohydrate (sc-255262)


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Graf, H., et al. 1994. Bioseparation. 4: 7-20. PMID: 7764588
3. Anfossi, L., et al. 2003. Ann Chim. 93: 499-512. PMID: 12911143
4. Mönch, S., et al. 2010. Anal. Biochem. 398: 150-160. PMID: 19903444
5. Teste, B., et al. 2010. Talanta. 81: 1703-1710. PMID: 20441961

Usage :
Filter solution through a 0.22 μm filter into a sterile flask. Solutions are stable when stored at 2-8°C for several months. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, then add the buffer after autoclaving.
Appearance :
Crystalline
Physical State :
Solid
Solubility :
Soluble in water (~1.3 M).
pH :
useful buffering range: pH 5.5 - 6.7
Storage :
Store at room temperature
Melting Point :
>300° C
Density :
1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.52 (Predicted)
pK Values :
pKa: 6.1 at 25°C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
QE3479500
PubChem CID :
78165
Merck Index :
14: 5902
MDL Number :
MFCD00149409
EC Number :
224-632-3
Beilstein Registry :
141319
SMILES :
C1COCCN1CCS(=O)(=O)O

Download SDS (MSDS)

Certificate of Analysis

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Rated 5 out of 5 by from Various publications cite the use of MES Various publications cite the use of MES, a zwitterionic buffer, as a running buffer for resolving very small proteins (ex: 13 kDa) on bis-tris gels. Also used in cation-exchange chromatography and as a biological buffer in plant cell cultures. MES solutions can be prepared to the desired pH by titrating the free acid with sodium hydroxide. -SCBT Publication Review
Date published: 2015-09-10
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