MeOSuc-AAPV-AMCA fluorogenic substrate used to assay elastase activity

MeOSuc-AAPV-AMC (CAS 72252-90-5)

MeOSuc-AAPV-AMC | CAS 72252-90-5 is rated 5.0 out of 5 by 1.
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Synonym: Neutrophil elastase substrate (fluorogenic); N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin; Elastase Substrate V
Application: A fluorogenic substrate used to assay elastase activity
CAS Number: 72252-90-5
Purity: ≥95%
Molecular Weight: 627.69
Molecular Formula: C31H41N5O9
* Refer to Certificate of Analysis for lot specific data (including water content).
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MeOSuc-AAPV-AMC is a fluorogenic substrate of neutrophil elastase. This compound can be used to assay elastase activity by spectrofluorometrically monitoring elastase-produced hydrolysis products. Assays of human leukocytes and porcine pancreatic elastase is described with use of MeOSuc-AAPV-AMC as a probe.


References

1. Castillo, M.J., et al. 1979. Anal. Biochem. 99: 53-64. PMID: 394626
2. Edwards, K.M., et al. 1999. J. Biol. Chem. 274: 30468-30473. PMID: 10521426
3. Coles, B., et al. 2002. Circ. Res. 91: 375-381. PMID: 12215485
4. Cloutier, S.M., et al. 2004. Eur. J. Biochem. 271: 607-613. PMID: 14728688

Physical State :
Solid
Sequence :
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amino-4-methylcoumarin
Solubility :
Soluble in DMSO (>3.2 mM), ethanol, water, and chloroform (50 mg/ml).
Storage :
Store at -20° C
Boiling Point :
~983.7° C at 760 mmHg (Predicted)
Density :
~1.3 g/cm3 (Predicted)
Refractive Index :
n20D 1.57
Fluoresence :
λex 380 nm, λem 460 nm in conditioned medium; λex 355 nm, λem 440 nm in Conditioned medium; λex 375 nm, λem 450 nm in Conditioned medium
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
4083261
MDL Number :
MFCD00077135
SMILES :
CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(C(C)C)NC(=O)C3CCCN3C(=O)C(C)NC(=O)C(C)NC(=O)CCC(=O)OC

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Certificate of Analysis

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MeOSuc-AAPV-AMC  Product Citations

See how others have used MeOSuc-AAPV-AMC. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 25365140  Budnjo, A. et al. 2014. J. Med. Chem. 57: 9396-408.

Citations 1 to 1 of 1 total

Hi, I was wondering which substrate is more specific for PR3 enzyme MeOSuc-AAPV-pNA or MeOSuc-AAPV-AMC?

Asked by: AAftab
Thank you for your question. MeOSuc-AAPV-pNA has been found to be a substrate for PR3, we do not have any literature supporting MeOSuc-AAPV-AMC being a highly sensitive substrate for PR3.
Answered by: Technical Support
Date published: 2017-04-05

Any recommendations for solubility in aqueous buffers?

Asked by: chemicalsmg
Thank you for your question. This chemical is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, first dissolve in DMF and then dilute with the aqueous buffer of choice. Solubility is approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-12
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Rated 5 out of 5 by from Various publications cite the use of MeOSuc Various publications cite the use of MeOSuc-AAPV-AMC to assay elastase activity by spectrofluorometrically monitoring elastase-produced hydrolysis products. -SCBT Publication Review
Date published: 2015-06-09
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