m-IgG lambda BP-HRP mouse IgG lambda binding protein (m-IgGλ BP) conjugated to horseradish peroxidase (HRP), for WB, IHC(P) and ELISA

m-IgGλ BP-HRP: sc-516132

m-IgGλ BP-HRP is rated 4.4 out of 5 by 13.
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Datasheets
    • mouse IgG lambda binding protein (m-IgGλ BP) conjugated to Horseradish Peroxidase (HRP)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [ECL]) and Immunohistochemistry (IHC)
    • Suitable for binding to mouse IgGλ light chain immunoglobulins comprising approximately 2% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGκ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For a Cruz Marker™ compatible mouse IgG lambda binding protein, use m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM) (Click here for datasheet)
    • For mouse IgGκ immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies we recommend m-IgGκ BP-HRP (sc-516102)
    • If you have an interest in testing a 10 µg sample of m-IgGλ BP-HRP (sc-516132) or m-IgGλ BP-HRP (Cruz Marker) (sc-516132-CM), our highly recommended recombinant alternatives to conventional secondary anti-mouse IgG HRP reagents, please contact Technical Service.
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We didn't know our primary antibody subtype ( kappaor lamda), which 2nd we should order?

Asked by: yuhong
Thank you for your question. The significant majority of mouse monoclonal IgG primary antibodies (98%) have a kappa light chain. And therefore we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for Western blotting or immunohistochemistry. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp Only approximately 2% of mouse IgG contains a lambda light chain, but for those subtypes we do offer a lambda-specific mouse IgG binding protein, m-IgGλ BP-HRP: sc-516132.
Answered by: Technical Support
Date published: 2018-09-13

I was looking for an anti-rat secondary antibody to be used in WB, but I couldn´t find it. I need the secondary antibody for the primary antibodies: sc-58605, sc-58607, and sc-58596. Can you give me an alternative?

Asked by: Marcia1
Thank you for your question. Unfortunately, we do not currently offer any secondary antibodies specific for detection of rat immunoglobulins. We are working on developing new products, but these are not yet available. We recommend using any conventional anti-rat IgG secondary antibodies for detection of the primary antibodies you have referenced above.
Answered by: Technical Support
Date published: 2017-01-11
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Rated 4 out of 5 by from Western blotting Use 1:5000 dilution and works great for mouse liver lysates
Date published: 2018-01-15
Rated 4 out of 5 by from Works well This antibody worked very well in several of my WB experiments.
Date published: 2017-12-19
Rated 5 out of 5 by from good result on WB The m-IgGλ BP-HRP antibody works well in Werstern Blot detection using Rat articular chondrocytes cell at 1:800. we got a clear protein band.
Date published: 2017-07-14
Rated 4 out of 5 by from Good AB It is good for WB and always get strong signaling at 1:5000.
Date published: 2017-07-06
Rated 5 out of 5 by from good It is very sensitive ,works very well in my experiments
Date published: 2017-07-06
Rated 5 out of 5 by from I would recommend this antibody I would recommend this antibody. It worked fine at concentration at 1:5000
Date published: 2017-06-01
Rated 4 out of 5 by from very good It works in my western blot at 1:5000,very sensitive.The antibodies can be reused for WB.
Date published: 2017-05-22
Rated 2 out of 5 by from we still like SC-2005,Sc-2030,Sc-2031 antibodies Not always working well. not as good as SC-2005,Sc-2030,SC-2031 antibodies
Date published: 2017-03-21
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