m-IgG kappa BP-PE mouse IgG kappa binding protein (m-IgGκ BP) conjugated to phycoerythrin (PE), for WB, IF, IHC(P) and FCM

m-IgGκ BP-PE: sc-516141

m-IgGκ BP-PE is rated 4.4 out of 5 by 14.
  • y_2019, m_7, d_18, h_24
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Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to phycoerythrin (PE)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-PE (sc-516141), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG PE reagents, please contact our Technical Service Department (or your local Distributor) for more information.
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Rated 3 out of 5 by from Average staining of Ku70 in human and mouse cells I tested this PE-tagged secondary antibody with a primary antibody (sc-56129) in flow cytometry. Stained 1 million HEK 293T cells and B16F10 with 1:50 dilution of the primary and 1:200 of the PE-tagged secondary. The resolution was not great, and the stain index was very small. It was better for the HEKs than the B16F10s.
Date published: 2018-06-18
Rated 4 out of 5 by from Worked well I got this antibody as a free sample. It worked well for immunofluorescence applications (1:100 dilution), however it does have a little bit of a background.
Date published: 2017-12-01
Rated 5 out of 5 by from good the fluorescence intensity presented in HGEC is appropriate
Date published: 2017-10-31
Rated 1 out of 5 by from Not optimized I got a free sample of this product and I have tested it for intracellular staining and surface staining as well, but unfortunately, there are lots of backgrounds so I can not interpret my data at all from that, even with 1:100 dilution. Also, I am using anti-gamma globin antibody as a primary antibody from your company.I would be happy if you could help me to solve this problem.
Date published: 2017-09-30
Rated 5 out of 5 by from useful the sample is very sensitive but may get a little background.
Date published: 2017-09-11
Rated 5 out of 5 by from Good antibody I got this antibody from SCBT as a free sample. It works well for mouse skeletal muscle.
Date published: 2017-08-23
Rated 4 out of 5 by from Good antibody for IF of rat ADSCs Great antibody work well in IF at 1:50 of rat ADSCs.
Date published: 2017-08-17
Rated 5 out of 5 by from good Very good second antibody, the specificity is good, the effect of dyeing is also very good
Date published: 2017-06-01
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