m-IgG kappa BP-HRP mouse IgG kappa binding protein (m-IgGκ BP) conjugated to horseradish peroxidase (HRP), for WB, IHC(P) and ELISA

m-IgGκ BP-HRP: sc-516102

m-IgGκ BP-HRP is rated 4.7 out of 5 by 295.
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Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP)
    • supplied at 200 µg in 0.5 ml volume
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for Western blotting (WB) and Immunohistochemistry (IHC)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
    • For a Cruz Marker™ compatible mouse IgG kappa binding protein, use m-IgGκ BP-HRP (Cruz Marker) (sc-516102-CM) (Click here for datasheet)
    • For mouse IgGλ immunoglobulins, comprising approximately 2% of mouse monoclonal antibodies; we recommend m-IgGλ BP-HRP (sc-516132)
    • If you have an interest in testing a 10 µg sample of m-IgGκ BP-HRP (sc-516102) or m-IgGκ BP-HRP (Cruz Marker) (sc-516102-CM), our highly recommended recombinant alternatives to conventional secondary anti-mouse IgG HRP reagents, please contact Technical Service.
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I used Mfn2 Antikörper(XX-1):sc-100560 with goat anti-mouse IgG-HRP sc-2005 (production stopped).Now I tried with m-IgGκ BP-HRP: sc-516102 as secondary AB but no signal was detected in WB (also not in positive contr.) Are any problems known with the AB?

Asked by: Jessika
Thank you for your question. This secondary antibody is compatible for use with the primary antibody you referenced. Please contact our Technical Service for support in troubleshooting the antibodies.
Answered by: Technical Support
Date published: 2018-11-30

Podrían decirme cuál es el origen de m-igGk BP-HRP?

Asked by: dmalcazar
Muchas gracias por su pregunta. Lamentablemente esa información es confidencial, la producción de estas proteínas de unión está basada en tecnología recombinante.
Answered by: Technical Support Europe
Date published: 2017-11-22

Does this product react with immunoglobulin used in IPs and then transferred prior to a western? (That is, what is the background when mouse IgG is used to ip the sample

Asked by: BrianSS
Thank you for your question. mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP), m-IgGκ BP-HRP: sc-516102, specifically binds to only mouse IgGκ light chain immunoglobulins. Therefore, if you used this for Western blot detection following IP you would not observe any binding to mouse IgG heavy chain.
Answered by: Technical Support
Date published: 2018-05-15

Hello, can m-IgGk BP-HRP (sc-516102) be used with mouse tissues in western blot and histochemistry without any background?  We usually suffer from endogenous mouse IgG in both the methods when we use monoclonal primary antibodies.

Asked by: Takashi
Thank you for your question. Yes, we do recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) in mouse tissues for both Western blotting and immunohistochemistry. This alternative secondary specifically binds to only the light chain of mouse IgG and does not have as much background as a conventional anti-mouse IgG secondary antibody which may be inherently "sticky" in tissues, and also bind to other mouse IgG epitopes. Below are links to our recommended protocols that reference use of our m-IgGκ BP-HRP: sc-516102. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-10-09

Hola, utilizando este anticuerpo para WB, es posible revelar con fosfatasa alcalina o que producto recomendarian?

Asked by: KarineVet
Muchas gracias por su pregunta. Este anticuerpos está conjugado a HRP, recomendamos como sustrato: Chemiluminescence Luminol Reagent (sc-2048) para Western Blott y DAB (sc-24982) para IHC (P). Si necesita cualquier información adicional, por favor, no dude en contactar directamente con nuestro Servicio de Atención Técnica: scbt@scbt.com
Answered by: Technical Support Europe
Date published: 2017-10-04

sc-516102 è utilizzabile in ELISA con THP(B-2) (sc-271022)?

Asked by: Danlu70
Grazie mille per il feedback. Il prodotto è fortemente raccomandato come alternativa ricombinante ai reagenti secondari convenzionali anti-mouse IgG, ed è garantito per l'utilizzo con sc-271022 per il rilevamento di THP di origine mouse, rat e human in WB, IF, IHC(P) e ELISA. La Santa Cruz Biotechnology garantisce i suoi prodotti per un anno dalla data d'acquisto.
Answered by: Technical Support Europe
Date published: 2017-10-04

Can this secondary antibody be used to detect mouse IgA antibody, provided that it has kappa light chain?

Asked by: Newt
Thank you for your question. Unfortunately this is not something we have tested, so we are not sure it will pick up IgA. It is definitely possible that it will, but we can not guarantee that it will. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-10-04

Hi will this secondary kappa light chain recognize a mouse monoclonal antibody isotype IgG2a

Asked by: taskl
Thank you for your question. Yes, this should be fine, the mouse IgG binding protein should pick pup the IgG2a primary antibodies. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-10-04
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Rated 5 out of 5 by from 抗体非常好 条带清晰,真不错。我跑的是hct116,p65,1:1000,希望对大家有所帮助。下次准备购买正装。
Date published: 2018-12-29
Rated 5 out of 5 by from great For mouse hippocampus worked well (WB) with 1:1000
Date published: 2018-12-18
Rated 4 out of 5 by from M ig GK BO HRp It really work well with breast cancer cell line i recommende it
Date published: 2018-12-18
Rated 1 out of 5 by from Not working well with some of mouse primary Abs It did work well with some primary antibodies (e.g., sc-136178), but did not work (no signal) with some of primary antibodies (e.g., sc-393031, sc-420). We are consulting with the technical service to resolve this issue.
Date published: 2018-10-26
Rated 5 out of 5 by from Works exactly as advertised Produces clean, crisp, publication quality western blots
Date published: 2018-09-14
Rated 5 out of 5 by from It worked great! It worked really well for primary mouse antibody at 1:2000 dilution without any background on nitrocelluose membrane. I highly recommend it
Date published: 2018-08-29
Rated 5 out of 5 by from A great secondary antibody It is a great seconday antiboy for WEstern blot as well as immunohistochemical staining .
Date published: 2018-08-27
Rated 5 out of 5 by from Great product, can also detect IgA m-IgGk BP-HRP worked great detecting IgA antibody (ATF1 C41-5.1) in WB (1:1000 dilution).
Date published: 2018-08-23
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