Luminol A chemiluminescence generating reagent for biological and metal cation assays

Luminol (CAS 521-31-3)

Luminol | CAS 521-31-3 is rated 5.0 out of 5 by 2.
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Synonym: 3-Aminophthalhydrazide
Application: A chemiluminescence generating reagent for biological and metal cation assays
CAS Number: 521-31-3
Purity: ≥97%
Molecular Weight: 177.16
Molecular Formula: C8H7N3O2
* Refer to Certificate of Analysis for lot specific data (including water content).
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Luminol is a reagent for the generation of chemiluminescence in biological assays including nitric oxide and myeloperoxidase assay. Luminol is converted to a dianion in basic solution, which is then oxidized with H2O2/Cu2+ to form N2 and an aminophthalate ion in an electronically excited state. The aminophthalate ion emits a photon of wavelength 424 nm.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (50 mg/ml), and 10% hot ammonia (100 mg/ml). Insoluble in water.
Storage :
Store at -20° C
Melting Point :
>300° C (lit.)
Boiling Point :
467.05° C (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.65 (Predicted)
Ki Data :
tRNA-guanine transglycosylase: Ki= 36 µM (Zymomonas mobilis )
pK Values :
pKa: 10.55 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
TH8890060
PubChem CID :
10638
Merck Index :
14: 5600
MDL Number :
MFCD00006890
EC Number :
208-309-4
Beilstein Registry :
383929
SMILES :
C1=CC2=C(C(=C1)N)C(=O)NNC2=O

Download SDS (MSDS)

Certificate of Analysis

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Luminol  Product Citations

See how others have used Luminol. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 15244513  Sengupta, A. et al. 2004 Apr-Jun. Asian Pac. J. Cancer Prev. 5: 126-132.

PMID: # 16235994  Ganguly, C. et al. 2005 Jul-Sep. Asian Pac. J. Cancer Prev. 6: 326-331.

Citations 1 to 2 of 2 total
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Rated 5 out of 5 by from Worked great on western blot Works great on western blot for chemiluminscent development.
Date published: 2017-08-04
Rated 5 out of 5 by from Various publications cite the use of luminol Various publications cite the use of luminol as a reagant of chemiluminescence iological assays. -SCBT Publication Review
Date published: 2015-01-23
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