Laemmli Sample Buffer (odor-free), 2X A sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system

Laemmli Sample Buffer (odor-free), 2X

Laemmli Sample Buffer (odor-free), 2X is rated 5.0 out of 5 by 1.
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Application: A sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system
* Refer to Certificate of Analysis for lot specific data (including water content).
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Laemmli Sample Buffer (odor-free), 2X is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The DTT reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.


References

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606

Usage :
Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
Formulation :
2X solution contains 4% SDS, 20% glycerol, 10% 400 mM DTT, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.
Physical State :
Liquid
pH :
~6.8
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Laemmli Sample Buffer (odor-free), 2X  Product Citations

See how others have used Laemmli Sample Buffer (odor-free), 2X. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 26893626  Gao, X. et al. 2016. Exp Ther Med. 11: 427-434.

PMID: # 26983346  Zhao, GX. et al. 2016. Biotech Histochem. 91: 269-76.

Citations 1 to 2 of 2 total
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Rated 5 out of 5 by from Various publications cite Laemmli Sample Various publications cite Laemmli Sample Buffer as a sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system. -SCBT Publication Review
Date published: 2015-07-15
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