Ig kappa chain Antibody (L1C1) recommended for detection of Ig κ chain of human origin by WB, IP and FCM

Ig κ chain Antibody (L1C1): sc-59265

Ig κ chain Antibody (L1C1) is rated 5.0 out of 5 by 4.
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Datasheets
    • Ig κ chain Antibody (L1C1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against B lymphoma cells of human origin
    • recommended for detection of Ig κ chain of human origin by WB, IP and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Ig κ chain (L1C1): sc-59265.
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How can Ig κ chain (L1C1): sc-59265 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-59265 PE) or fluoroscein (sc-59265 FITC).
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Great for Carcinoma Detection Received this as a sample. We used this on Human FFPE carcinoma tissues and got good staining. Use with sodium citrate pH 6.0 at 1:100.
Date published: 2017-11-29
Rated 5 out of 5 by from Favorable signal seen against human Ig Favorable signal seen against human Ig chain transfected 293T and human PBL whole cell lysates. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Good bands produced when using this antibody Good bands produced when using this antibody against Raji and GA-10 whole cell lysates. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Produced nice indirect FCM data for human Produced nice indirect FCM data for human peripheral blood leukocytes stained with Ig k Chain . -SCBT QC
Date published: 2013-01-17
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