Ig κ chain Antibody (L1C1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against B lymphoma cells of human origin
recommended for detection of Ig κ chain of human origin by WB, IP and FCM
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ig κ chain (L1C1): sc-59265.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Antibody producing cells of the immune system require multiple rearrangements of immunoglobulin (antibody, Ig) genes. Immunoglobulins are four-chain, Y-shaped, monomeric structures of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. Immunoglobulins in vertebrates help to remove non-self molecules or cells (antigens) by recognizing and binding to the antigen and carrying out effector functions that activate the immune system. Variable genetic combinations of the five heavy chain classes (M, D, G, E and A) and the two light chain isotypes, κ and λ, confer the role of an antibody. The variable region genes encoding immunoglobulin κ and λ chains are assembled from three DNA segments, the V, C and J genes. Human κ light chain genes map to chromosome 2 and the human λ light chain genes map to chromosome 22. κ gene recombination can precede λ gene recombination during B cell ontogeny and only a single light chain type is expressed in individual B cells. Antibodies in camels and sharks can lack light chain, suggesting that light chain may not be essential for antigen binding in some vertebrates.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 5 out of
Great for Carcinoma DetectionReceived this as a sample. We used this on Human FFPE carcinoma tissues and got good staining. Use with sodium citrate pH 6.0 at 1:100.
Date published: 2017-11-29
Rated 5 out of
Favorable signal seen against human IgFavorable signal seen against human Ig chain transfected 293T and human PBL whole cell lysates. -SCBT QC
Date published: 2015-04-19
Rated 5 out of
Good bands produced when using this antibodyGood bands produced when using this antibody against Raji and GA-10 whole cell lysates. -SCBT QC
Date published: 2015-03-05
Rated 5 out of
Produced nice indirect FCM data for humanProduced nice indirect FCM data for human peripheral blood leukocytes stained with Ig k Chain . -SCBT QC
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