IDO Antibody (mIDO-48) recommended for detection of IDO of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

IDO Antibody (mIDO-48): sc-53978

IDO Antibody (mIDO-48) is rated 5.0 out of 5 by 16.
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Datasheets
    • IDO Antibody (mIDO-48) is a rat monoclonal IgG2b provided at 200 µg/ml
    • raised against recombinant full length IDO of mouse origin
    • recommended for detection of IDO of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact us to receive a FREE 10 µg sample of IDO (mIDO-48): sc-53978.
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Can you recommend a positive control to use with IDO Antibody (mIDO-48): sc-53978 for Western blot?

Asked by: jenni
We recommend MOLT-4: sc-2233, Raji: sc-364236 or or human lymph node tissue extract: sc-363768 whole cell lysates to use as positive controls as we have Western blot data showing specific detection of IDO in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-09

Hi, we found three bands (close to 45 KDa) on our western blot after using this antibody (sample from rat brain). I wonder if it is different isoforms of IDO or something else. Was the full lenght protein it was raised against IDO1 or IDO2?

Asked by: JulianoK
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Rated 5 out of 5 by from CLEAR BANDS The bands are clear. But I got 2 at the target position which quite confused me. Has anyone met this before?
Date published: 2017-12-20
Rated 5 out of 5 by from 功能很出色 申请了抗体小样,提取的是回肠末端蛋白,western跑出来条带比较清晰,基本没看到杂带,总体性能还不错。
Date published: 2017-09-07
Rated 5 out of 5 by from excellent I used in lung cancer cell line 2LL for WB and IF, showing good results.
Date published: 2017-08-22
Rated 5 out of 5 by from Work Well on WB We used this IDO Antibody for RAW264.7 cell by WB and the results were specific and strong.
Date published: 2017-08-03
Rated 5 out of 5 by from very useful IL10 antibody effect is good, immunohistochemical staining uniform, positive coloring significantly. Recommend you to buy, inexpensive, with 1:100 with the very good.
Date published: 2017-07-21
Rated 5 out of 5 by from Mouse & Human tissues look great! Last week we worked out this antibody using human and mouse normal and abnormal tissues. Dilution 1:100, HIER sodium citrate pH 6.0. All tissues are beautiful!
Date published: 2017-06-20
Rated 5 out of 5 by from Great Clean blot with single strong band at expected in mouse placenta tissue extract
Date published: 2017-05-19
Rated 5 out of 5 by from WB This antibody could be used to de WB, and the result is very good.
Date published: 2017-04-06
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