Hygromycin B solution An antibiotic often used for selection of cells transfected with E. coli hygromycin resistance gene.

Hygromycin B solution (CAS 31282-04-9)

Hygromycin B solution | CAS 31282-04-9 is rated 5.0 out of 5 by 4.
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Synonym: Hygrovetine; D-streptamine
Application: An antibiotic often used for selection of cells transfected with E. coli hygromycin resistance gene.
CAS Number: 31282-04-9
Purity: >90%
Molecular Weight: 527.52
Molecular Formula: C20H37N3O13
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Hygromycin B is an aminoglycoside antibiotic substance naturally produced by Streptomyces hygroscopicus, which also shows anthelmintic activity. Hygromycin B has been shown to effectively inhibit translocation of mRNA and tRNAs on the ribosome in both bacteria and eukaryotes and also has a subtle effect on decoding fidelity as it slightly increases aminoacyl tRNA (A-site tRNA) affinity. Suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.


References

1. González, A., et al. 1978. Biochim. Biophys. Acta. 521: 459-469. PMID: 367435
2. Rao, R.N., et al. 1983. Antimicrob. Agents Chemother. 24: 689-695. PMID: 6318654
3. Cullen, D., et al. 1987. Gene. 57: 21-26. PMID: 3322945
4. Perlin, D.S., et al. 1988. J. Biol. Chem. 263: 18118-18122. PMID: 3056938
5. Kronstad, J.W., et al. 1989. Gene. 79: 97-106. PMID: 2673937
6. Perlin, D.S., et al. 1989. J. Biol. Chem. 264: 21857-21864. PMID: 2532214
7. Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9: 1965-1968. PMID: 2546056
8. Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 34: 1915-1920. PMID: 1963287
9. Bilang, R., et al. 1991. Gene. 100: 247-250. PMID: 1647356
10. Dean, N., 1995. Proc. Natl. Acad. Sci. U.S.A. 92: 1287-1291. PMID: 7877969

Usage :
• This product is provided as 20 mL of a sterile filtered solution in PBS.


• Appearance: Solution color can vary from light yellow to a dark brown or caramel color. Concentrated Hygromycin solution tends to have a darker appearance.


• The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. A titration experiment for each cell type should be performed to determine the amount of Hygromycin B needed to kill non-transfected cells.


• Recommended concentrations for the selection of resistant cells is 25-1000 μg/ml. Commonly used concentrations for selection are 200 μg/ml for mammalian cells, 20-200 μg/ml for plant cells & bacteria cells and 200-1000 μg/ml for fungi. Your optimum concentration should be tested experimentally.


• Control cells should die within 5-7 days after addition of the antibiotic allowing colonies of resistant cells to form by 10–14 days.
Formulation :
50 mg/ml in PBS
Appearance :
Solution
Physical State :
Liquid
Derived From :
Streptomyces hygroscopicus
Solubility :
Soluble in water, methanol, buffer solution, and ethanol.
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
RTECS :
WK2130000
Transport :
UN 2810, Class 6.1, Packing group II
PubChem CID :
35766
Merck Index :
14: 4852
MDL Number :
MFCD06795479
EC Number :
250-545-5
SMILES :
CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N

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Hygromycin B solution  Product Citations

See how others have used Hygromycin B solution. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 17 total

PMID: # 28870912  Pan, PJ. et al. 2017. Anticancer Res. 37: 4911-4918.

PMID: # 25271153  Kannike, K. et al. 2014. J. Biol. Chem. 289: 32845-57.

PMID: # 24606125  Eigler, T. et al. 2014. Mol. Endocrinol. 28: 554-64.

PMID: # 19468300  Wolfe, BA. et al. 2009. PLoS Biol. 7: -.

PMID: # 17362967  Laurentino, EC. et al. 2007. Int. J. Parasitol. 37: 735-742.

PMID: # 17995645  Lu, Y. et al. 2007. J. Food Sci. 72: M228-M232.

PMID: # 17470460  Kaimori, JY. et al. 2007. Hum. Mol. Genet. 16: 942-56.

PMID: # 17242188  Niida, H. et al. 2007. Mol. Cell. Biol. 27: 2572-2581.

PMID: # 17392175  Cui, H. et al. 2007. Am. J. Pathology. 170: 1370-1378.

PMID: # 16397136  Mankad, A. et al. 2006. Blood. 107: 3084-3090.

Citations 1 to 10 of 17 total

Is this product sterile?

Asked by: hawkeye11
Thank you for your question. This chemical, Hygromycin B solution (CAS 31282-04-9), is considered sterile.
Answered by: Chemical Support 1
Date published: 2018-09-16
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Rated 5 out of 5 by from Works well in our cell culture studies Works well in our cell culture studies, good quality.
Date published: 2015-11-04
Rated 5 out of 5 by from Worked fine Worked fine.
Date published: 2015-09-23
Rated 5 out of 5 by from We purchased this product several times We purchased this product several times. It works consistently well. The price is reasonable.
Date published: 2015-03-17
Rated 5 out of 5 by from Hygromycin B was studied in regards to it Hygromycin B was studied in regards to it structure as a basis for antibiotic functionality via interaction with the 30S Ribosomal unit (PubMed ID 11163189). -SCBT Publication Review
Date published: 2015-03-17
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