Histone H1 Antibody (H-2) recommended for detection of all histone H1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA

Histone H1 Antibody (H-2): sc-393358

Histone H1 Antibody (H-2) is rated 4.3 out of 5 by 7.
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Datasheets
    • Histone H1 Antibody (H-2) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 22-47 near the N-terminus of Histone H1 of human origin
    • recommended for detection of all histone H1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-393358 X, 200 µg/0.1 ml)
    • blocking peptide, sc-393358 P
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Histone H1 (H-2): sc-393358.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Histone H1 Antibody (H-2) sc-393358 200 µg/ml $279.00
Histone H1 Antibody (H-2) X sc-393358 X 200 µg/0.1 ml $279.00
Histone H1 Antibody (H-2) AC sc-393358 AC 500 µg/ml, 25% agarose $366.00
Histone H1 Antibody (H-2) HRP sc-393358 HRP 200 µg/ml $279.00
Histone H1 Antibody (H-2) FITC sc-393358 FITC 200 µg/ml $292.00
Histone H1 Antibody (H-2) PE sc-393358 PE 200 µg/ml $303.00
Histone H1 Antibody (H-2) Alexa Fluor® 488 sc-393358 AF488 200 µg/ml $314.00
Histone H1 Antibody (H-2) Alexa Fluor® 546 sc-393358 AF546 200 µg/ml $314.00
Histone H1 Antibody (H-2) Alexa Fluor® 594 sc-393358 AF594 200 µg/ml $314.00
Histone H1 Antibody (H-2) Alexa Fluor® 647 sc-393358 AF647 200 µg/ml $314.00
Histone H1 Antibody (H-2) Alexa Fluor® 680 sc-393358 AF680 200 µg/ml $314.00
Histone H1 Antibody (H-2) Alexa Fluor® 790 sc-393358 AF790 200 µg/ml $314.00
Histone H1 (H-2) Blocking Peptide sc-393358 P 100 µg/0.5 ml $61.00

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Histone H1 Antibody (H-2)Product Citations

See how others have used Histone H1 Antibody (H-2). Click on the entry to view the PubMed entry .

Citations 1 to 7 of 7 total

PMID: # 29470442  Kim, KM. et al. 2018. Molecules. 23:

PMID: # 29705243  Van Nieuwenhove, E. et al. 2018. J. Allergy Clin. Immunol. 142: 699-702.e12.

PMID: # 28470764  Antognelli, C. et al. 2017. Mol. Carcinog. 56: 2112-2126.

PMID: # 26228975  Chen, H. et al. 2016. Inflammation. 39: 39-46.

PMID: # 25584861  Szerlong, HJ. et al. 2015. Journal of molecular biology. 427: 2056-71.

PMID: # 24984764  Pavlosky, A. et al. 2014. Am. J. Transplant. 14: 1778-90.

PMID: # 25173814  Ishaq, M. et al. 2014. Biochimica et biophysica acta. 1843: 2827-37.

Citations 1 to 7 of 7 total

When should the TransCruz reagent sc-393358 X be used instead of the standard form sc-393358?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 7
Date published: 2017-01-13

What secondary is best to use with Histone H1 (H-2): sc-393358?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Histone H1 (H-2):sc-393358, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 1 out of 5 by from Not good for IF Weak signal for IF in hunam HT1080 cells at 1:100 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good for WB Strong single band - worked well in nuclear fractionation WB.
Date published: 2017-09-13
Rated 5 out of 5 by from Works great for Western Blot. Used at a concentration of 1:2000 for Western blot with mouse tissues. Worked very well, clean blot and a single band. I would recommend!
Date published: 2017-09-01
Rated 5 out of 5 by from Superb !! Great product for western blot applications; strong signal
Date published: 2017-01-20
Rated 5 out of 5 by from good in WB This Histone H1 antibody shows specific strong bands in WB.
Date published: 2017-01-12
Rated 4 out of 5 by from Potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~32-33 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using A-431, LNCaP, HeLa and HL-60 whole cell lysates and Jurkat nuclear extract. -SCBT QC
Date published: 2014-07-05
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