HEPES hemisodium salt A buffering component

HEPES hemisodium salt (CAS 103404-87-1)

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Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt
Application: A buffering component
CAS Number: 103404-87-1
Purity: ≥99%
Molecular Weight: 249.30
Molecular Formula: C8H17.5N2O4S•0.5Na
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES hemisodium salt (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt) may be used in the preparation of HEPES buffer, which has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5.


1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Usage :
This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
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For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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