HEPES Buffer Solution (1M) A zwitterionic, all-purpose "Good's" buffer for use in biological research

HEPES Buffer Solution (1M) (CAS 7365-45-9)

HEPES Buffer Solution (1M) | CAS 7365-45-9 is rated 5.0 out of 5 by 1.
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Application: A zwitterionic, all-purpose "Good's" buffer for use in biological research
CAS Number: 7365-45-9
Molecular Weight: 238.3 g/L
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.8 to 8.2. Contains 238.3 g/L prepared in distilled water.
Also available: HEPES sodium salt solution (1M) (sc-300789)


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Usage :
HEPES Buffer Solution (1M) is prepared with high-quality reagents and 0.2 μm filtered; however it has not been evaluated for use in cell culture. Please employ appropriate sterilization techniques for the intended application.
Formulation :
1M
Appearance :
Solution
pH :
7.2 to 7.5
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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HEPES Buffer Solution (1M)  Product Citations

See how others have used HEPES Buffer Solution (1M). Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 19454526  Guimarães, ET. et al. 2009. J. Antimicrob. Chemother. 64: 84-7.

PMID: # 16531090  Guimarães, ET. et al. 2006. Microbes Infect. 8: 1219-26.

Citations 1 to 2 of 2 total
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Rated 5 out of 5 by from Various publications cite the use of HEPES Various publications cite the use of HEPES Buffer Solution in protein extraction protocols and various antigen-antibody binding assays. -SCBT Publication Review
Date published: 2015-04-13
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