HEPES An N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5

HEPES (CAS 7365-45-9)

HEPES | CAS 7365-45-9 is rated 5.0 out of 5 by 4.
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Synonym: N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid]; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Application: An N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5
CAS Number: 7365-45-9
Purity: ≥99%
Molecular Weight: 238.31
Molecular Formula: C8H18N2O4S
* Refer to Certificate of Analysis for lot specific data (including water content).
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HEPES (4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid) has been described as one of the best all-purpose buffers available for use in biological research. The molecule is zwitterionic at most biological pHs and is most effective as a buffer at pH 6.0 to 8.5. May be used in isoelectric focusing applications and as a buffer in the quantitative and selective measurement of antigen-antibody reactions. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. Not recommended for use with the Folin-Ciocalteu protein assay.


References

1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Medzon, E.L. and Gedies, A. 1971. Can. J. Microbiol. 17: 651-653. PMID: 5104240
3. Good, N.E. and Izawa, S. 1972. Meth. Enzymol. 24: 53-68. PMID: 4206745
4. Himmel, H.M., et al. 1987. J. Clin. Chem. Clin. Biochem. 25: 909-913. PMID: 3127532
5. Stoscheck, C.M. 1990. Meth. Enzymol. 182: 50-68. PMID: 2314256

Usage :
• Recommended working concentration: 10-25 mM

• A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with sodium hydroxide or potassium hydroxide to the pH desired. Alternatively, equimolar concentrations of HEPES and of sodium HEPES (sc-216092) can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH.
• This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (1 M) at 20 °C.
Storage :
Store at room temperature
Melting Point :
234° C (dec.)
Boiling Point :
210-215° C
Density :
1.33 g/cm3 (Predicted)
Refractive Index :
n20D 1.54 (Predicted)
pK Values :
pKa: 7.5, pKb: 6.52
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
TL6809000
PubChem CID :
23830
Merck Index :
14: 4654
MDL Number :
MFCD00006158
EC Number :
230-907-9
Beilstein Registry :
883043
SMILES :
C1CN(CC[NH+]1CCO)CCS(=O)(=O)[O-]

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Certificate of Analysis

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HEPES  Product Citations

See how others have used HEPES. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 19 total

PMID: # 27572343  Guo, T. et al. 2016. Sci Rep. 6: 32244.

PMID: # 25972601  Zacarias-Fluck, MF. et al. 2015. Journal of the National Cancer Institute. 107:

PMID: # 26075403  Vicario, R. et al. 2015. PloS one. 10: e0129876.

PMID: # 23068779  Guimarães, ET. et al. 2013. Cell transplantation. 22: 2345-54.

PMID: # 21683341  Guimarães, ET. et al. 2011. Archives of oral biology. 56: 1247-55.

PMID: # 19454526  Guimarães, ET. et al. 2009. J. Antimicrob. Chemother. 64: 84-7.

PMID: # 16531090  Guimarães, ET. et al. 2006. Microbes Infect. 8: 1219-26.

PMID: # 11306489  Hideshima, T. et al. 2001. Cancer Res. 61: 3071-3076.

PMID: # 10713049  Sun, X. et al. 2000. J. Biol. Chem. 275: 7466-7469.

PMID: # 10082535  Hinz, M. et al. 1999. Mol. Cell. Biol. 19(4): 2690-2698.

Citations 1 to 10 of 19 total

Is this product Pyrogen free?

Asked by: hawkeye11
This chemical, HEPES (CAS 7365-45-9), is not tested for Pyrogens.
Answered by: Chemical Support 1
Date published: 2017-03-09
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Rated 5 out of 5 by from Sehr gut! Sehr zufrieden mit Ihren Produkten und Dienstleistungen
Date published: 2018-03-12
Rated 5 out of 5 by from Works wells I removed from competitor to this product and works well for less.
Date published: 2017-05-23
Rated 5 out of 5 by from Great quality I've been buying this product from Santacruz for the last couple of years. Its quality is excellent and the price reasonable.
Date published: 2017-05-21
Rated 5 out of 5 by from Hideshima Hideshima, et. al. (PubMed ID 11306489) utilized HEPES in their hypotonic lysis buffer used to lyse BMSC's. -SCBT Publication Review
Date published: 2015-05-10
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